Animal-free heme production for artificial meat in Corynebacterium glutamicum via systems metabolic and membrane engineering
- Authors
- Ko, Young Jin; Kim, Minhye; You, Seung Kyou; Shin, Sang Kyu; Chang, Joonhee; Choi, Hae Jin; Jeong, Wu-Young; Lee, Myeong-Eun; Hwang, Dong-Hyeok; Han, Sung Ok
- Issue Date
- 7월-2021
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Heme; Corynebacterium glutamicum; Systems metabolic engineering; Noncanonical heme biosynthesis pathway; Membrane engineering
- Citation
- METABOLIC ENGINEERING, v.66, pp.217 - 228
- Indexed
- SCIE
SCOPUS
- Journal Title
- METABOLIC ENGINEERING
- Volume
- 66
- Start Page
- 217
- End Page
- 228
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/127804
- DOI
- 10.1016/j.ymben.2021.04.013
- ISSN
- 1096-7176
- Abstract
- Recently, heme has attracted much attention as a main ingredient that mimics meat flavor in artificial meat in the food industry. Here, we developed Corynebacterium glutamicum capable of high-yield production of heme with systems metabolic engineering and modification of membrane surface. The combination of two precursor pathways based on thermodynamic information increased carbon flux toward heme and porphyrin intermediate biosynthesis. The co-overexpression of genes involved in a noncanonical downstream pathway and the gene encoding the transcriptional regulator DtxR significantly enhanced heme production. The overexpression of the putative heme exporters, knockout of heme-binding proteins, modification of the cell wall by chemical treatment, and reduction of intermediate UP III substantially improved heme secretion. The fed-batch fermentation showed a maximum heme titer of 309.18 +/- 16.43 mg l- 1, including secreted heme of 242.95 +/- 11.45 mg l- 1, a yield on glucose of 0.61 mmol mol-1, and productivity of 6.44 mg l- 1h- 1, which are the highest values reported to date. These results demonstrate that engineered C. glutamicum can be an attractive cell factory for animal-free heme production.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.