Development and Validation of Enzyme-Linked Immunosorbent Assay for Group B Streptococcal Polysaccharide Vaccine
DC Field | Value | Language |
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dc.contributor.author | Jang, A-Yeung | - |
dc.contributor.author | Choi, Min-Joo | - |
dc.contributor.author | Zhi, Yong | - |
dc.contributor.author | Ji, Hyun-Jung | - |
dc.contributor.author | Noh, Ji-Yun | - |
dc.contributor.author | Yoon, Jin-Gu | - |
dc.contributor.author | Cheong, Hee-Jin | - |
dc.contributor.author | Kim, Woo-Joo | - |
dc.contributor.author | Seo, Ho-Seong | - |
dc.contributor.author | Song, Joon-Young | - |
dc.date.accessioned | 2021-11-19T01:40:27Z | - |
dc.date.available | 2021-11-19T01:40:27Z | - |
dc.date.created | 2021-08-30 | - |
dc.date.issued | 2021-06 | - |
dc.identifier.issn | 2076-393X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/127939 | - |
dc.description.abstract | Streptococcus agalactiae (group B Streptococcus, GBS) is a leading cause of neonatal sepsis and meningitis in infants. Limitations of prenatal GBS screening and intrapartum antibiotic prophylaxis render developing GBS vaccines a high priority. In this study, we developed an enzyme-linked immunosorbent assay (ELISA) for the practical and large-scale evaluation of GBS capsular polysaccharide (PS) vaccine immunogenicity against three main serotypes, Ia, III, and V. GBS-ELISA was developed and subsequently validated using a standardized curve-fitting four-parameter logistic method. Specificity was measured using adsorption of serum with homologous and heterologous PS. Homologous adsorption showed a >= 75% inhibition of all three serotypes, whereas with heterologous PS, IgG GBS-ELISA inhibited only <= 25% of serotypes III and V. However, with serotype Ia, IgG antibody levels decreased by >50%, even after adsorption with heterologous PS (III or V). In comparison, the inhibition opsonophagocytic killing assay (OPA) of serotypes Ia GBS exhibited a reduction in opsonophagocytic activity of only 20% and 1.1% for serotypes III and V GBS, respectively. The precision of the GBS-ELISA was assessed in five independent experiments using four serum samples. The coefficient of variation was <5% for all three serotypes. This standardized GBS-ELISA would be useful for GBS vaccine development and its evaluation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | QUANTITATIVE ESTIMATION | - |
dc.subject | CONJUGATE VACCINE | - |
dc.subject | SIALIC ACIDS | - |
dc.subject | IMMUNOGENICITY | - |
dc.subject | INFANTS | - |
dc.subject | DISEASE | - |
dc.subject | SAFETY | - |
dc.subject | HEALTHY | - |
dc.subject | WOMEN | - |
dc.title | Development and Validation of Enzyme-Linked Immunosorbent Assay for Group B Streptococcal Polysaccharide Vaccine | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Noh, Ji-Yun | - |
dc.identifier.doi | 10.3390/vaccines9060545 | - |
dc.identifier.scopusid | 2-s2.0-85107182983 | - |
dc.identifier.wosid | 000667869200001 | - |
dc.identifier.bibliographicCitation | VACCINES, v.9, no.6 | - |
dc.relation.isPartOf | VACCINES | - |
dc.citation.title | VACCINES | - |
dc.citation.volume | 9 | - |
dc.citation.number | 6 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | QUANTITATIVE ESTIMATION | - |
dc.subject.keywordPlus | CONJUGATE VACCINE | - |
dc.subject.keywordPlus | SIALIC ACIDS | - |
dc.subject.keywordPlus | IMMUNOGENICITY | - |
dc.subject.keywordPlus | INFANTS | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | SAFETY | - |
dc.subject.keywordPlus | HEALTHY | - |
dc.subject.keywordPlus | WOMEN | - |
dc.subject.keywordAuthor | Streptococcus agalactiae | - |
dc.subject.keywordAuthor | capsular polysaccharide | - |
dc.subject.keywordAuthor | enzyme-linked immunosorbent assay | - |
dc.subject.keywordAuthor | validation | - |
dc.subject.keywordAuthor | vaccine | - |
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