Switchable stimulated Raman scattering microscopy with photochromic vibrational probes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ao, Jianpeng | - |
dc.contributor.author | Fang, Xiaofeng | - |
dc.contributor.author | Miao, Xianchong | - |
dc.contributor.author | Ling, Jiwei | - |
dc.contributor.author | Kang, Hyunchul | - |
dc.contributor.author | Park, Sungnam | - |
dc.contributor.author | Wu, Changfeng | - |
dc.contributor.author | Ji, Minbiao | - |
dc.date.accessioned | 2021-11-19T14:40:30Z | - |
dc.date.available | 2021-11-19T14:40:30Z | - |
dc.date.created | 2021-08-30 | - |
dc.date.issued | 2021-05-25 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/128004 | - |
dc.description.abstract | Photochromic probes with reversible fluorescence have revolutionized the fields of single molecule spectroscopy and super-resolution microscopy, but lack sufficient chemical specificity. In contrast, Raman probes with stimulated Raman scattering (SRS) microscopy provides superb chemical resolution for super-multiplexed imaging, but are relatively inert. Here we report vibrational photochromism by engineering alkyne tagged diarylethene to realize photo-switchable SRS imaging. The narrow Raman peak of the alkyne group shifts reversibly upon photoisomerization of the conjugated diarylethene when irradiated by ultraviolet (UV) or visible light, yielding "on" or "off" SRS images taken at the photoactive Raman frequency. We demonstrated photo-rewritable patterning and encryption on thin films, painting/erasing of cells with labelled alkyne-diarylethene, as well as pulse-chase experiments of mitochondria diffusion in living cells. The design principle provides potentials for super-resolution microscopy, optical memories and switches with vibrational specificity. Probes with reversible fluorescence are useful in super-resolution microscopy, but lack sufficient chemical specificity. Here, the authors engineer alkyne tagged diarylethene to realize photo-switchable stimulated Raman scattering probes with high chemical resolution, for applications in living cells. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE RESEARCH | - |
dc.subject | FLUORESCENCE | - |
dc.subject | SENSITIVITY | - |
dc.subject | MOLECULES | - |
dc.subject | MEMORIES | - |
dc.subject | EMISSION | - |
dc.subject | DYNAMICS | - |
dc.subject | BREAKING | - |
dc.subject | PROTEIN | - |
dc.subject | CELLS | - |
dc.subject | LIMIT | - |
dc.title | Switchable stimulated Raman scattering microscopy with photochromic vibrational probes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Sungnam | - |
dc.identifier.doi | 10.1038/s41467-021-23407-2 | - |
dc.identifier.scopusid | 2-s2.0-85106760594 | - |
dc.identifier.wosid | 000658773300012 | - |
dc.identifier.bibliographicCitation | NATURE COMMUNICATIONS, v.12, no.1 | - |
dc.relation.isPartOf | NATURE COMMUNICATIONS | - |
dc.citation.title | NATURE COMMUNICATIONS | - |
dc.citation.volume | 12 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | FLUORESCENCE | - |
dc.subject.keywordPlus | SENSITIVITY | - |
dc.subject.keywordPlus | MOLECULES | - |
dc.subject.keywordPlus | MEMORIES | - |
dc.subject.keywordPlus | EMISSION | - |
dc.subject.keywordPlus | DYNAMICS | - |
dc.subject.keywordPlus | BREAKING | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | LIMIT | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.