Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Raman spectroscopy reveals alteration of spore compositions under different nutritional conditions in Lysinibacillus boronitolerans YS11

Authors
Ryu, YoungungHong, MinyoungKim, Soo BinLee, Tae KwonPark, Woojun
Issue Date
5월-2021
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Keywords
bacterial spore; heat-resistance; UV-resistance; raman spectroscopy; spore germination
Citation
JOURNAL OF MICROBIOLOGY, v.59, no.5, pp.491 - 499
Indexed
SCIE
SCOPUS
KCI
Journal Title
JOURNAL OF MICROBIOLOGY
Volume
59
Number
5
Start Page
491
End Page
499
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/128156
DOI
10.1007/s12275-021-0679-6
ISSN
1225-8873
Abstract
Little is known about final spores components when bacteria undergo sporulation under different nutrient conditions. Different degrees of resistance and germination rates were observed in the three types of spores of Lysinibacillus boronitolerans YS11 (SD, Spores formed in Difco sporulation medium (TM); SC and SF, Spores formed in an agricultural byproduct medium with 10 mM CaCl2 and with 10 mM FeSO4, respectively). Stronger UV resistance was recorded for SF with 1.8-2.3-fold greater survival than SC and SD under UV treatment. The three spore types showed similar heat resistances at 80 degrees C, but survival rates of SC and SD were much higher (similar to 1,000 times) than those of SF at 90 degrees C. However, germination capacity of SF was 20% higher than those of SD and SC on Luria-Bertani agar plates for 24 h. SF germinated more rapidly in a liquid medium with high NaCl concentrations than SC and SD, but became slower under alkaline conditions. Raman spectroscopy was used to analyze the heterogeneities in the three types of vegetative cells and their spores under different nutritional conditions. Exponentially grown-each vegetative cells had different overall Raman peak values. Raman peaks of SC, SD, and SF also showed differences in adenine and amide III compositions and nucleic acid contents. Our data along with Raman spectroscopy provided the evidence that spores formed under under different growth conditions possess very different cellular components, which affected their survival and germination rates.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Life Sciences and Biotechnology > Division of Environmental Science and Ecological Engineering > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Park, Woo jun photo

Park, Woo jun
생명과학대학 (환경생태공학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE