The Dopaminergic Neuronal System Regulates the Inflammatory Status of Mouse Lacrimal Glands in Dry Eye Disease
DC Field | Value | Language |
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dc.contributor.author | Ji, Yong Woo | - |
dc.contributor.author | Kang, Hyun Goo | - |
dc.contributor.author | Song, Jong Suk | - |
dc.contributor.author | Jun, Ji Won | - |
dc.contributor.author | Han, Kyusun | - |
dc.contributor.author | Kim, Tae-im | - |
dc.contributor.author | Seo, Kyoung Yul | - |
dc.contributor.author | Lee, Hyung Keun | - |
dc.date.accessioned | 2021-11-22T11:40:21Z | - |
dc.date.available | 2021-11-22T11:40:21Z | - |
dc.date.created | 2021-08-30 | - |
dc.date.issued | 2021-04 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/128348 | - |
dc.description.abstract | PURPOSE. Comparison of the parasympathetic and sympathetic neurons, including the dopaminergic neural system, in dry eye (DE)-induced pathophysiology has not been elucidated well. This study investigated the presence of dopamine receptors (DRs) and their functional roles in the lacrimal glands (LGs) of DE-induced mice. METHODS. After DE was induced in B6 mice for 2 weeks, the expression of tyrosine hydroxylase (TH), dopamine, and DRs (DR1, DR2, etc.) in the LGs and corneas were measured by quantitative RT-PCR, immunoblot, and ELISA. Using flow cytometry and ELISA, immune cell infiltration and inflammatory cytokine expression were determined in DE-induced LGs with or without DR blockers, SCH-23390 (DR1i), or melperone (DR2i). Corneal erosion scores were also investigated. RESULTS. The mRNA and protein levels of TH significantly increased in DE-induced LGs. The dopamine concentration of LGs was 9.51 pmol in DE (versus naive: 1.39 pmol; P < 0.001). Both DR1 and DR2 mRNA expression were significantly enhanced in desiccating stress compared with those in naive (3.7- and 2.1-fold, P < 0.001). Interestingly, DR1 and DR2 immunostaining patterns stained independently in DE-induced LGs. CD3(+) and CD19(+) cell infiltration was significantly increased by DR2i (P < 0.001) but not by DR1i. Furthermore, IFN-gamma, IL-17, and TNF-alpha were significantly upregulated by DR2i compared with the blow-only condition. The severity of corneal erosion and inflammation was also aggravated by DR2i. CONCLUSIONS. Upregulation of DR1 and DR2 was observed in DE-induced mouse LGs. As the inflammatory conditions are aggravated by the inhibition of DRs, especially DR2, their activity may be an important factor preserving ocular surface homeostasis. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | - |
dc.title | The Dopaminergic Neuronal System Regulates the Inflammatory Status of Mouse Lacrimal Glands in Dry Eye Disease | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Jong Suk | - |
dc.identifier.doi | 10.1167/iovs.62.4.14 | - |
dc.identifier.scopusid | 2-s2.0-85104335823 | - |
dc.identifier.wosid | 000639527200014 | - |
dc.identifier.bibliographicCitation | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, v.62, no.4 | - |
dc.relation.isPartOf | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | - |
dc.citation.title | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | - |
dc.citation.volume | 62 | - |
dc.citation.number | 4 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Ophthalmology | - |
dc.relation.journalWebOfScienceCategory | Ophthalmology | - |
dc.subject.keywordAuthor | dry eye disease | - |
dc.subject.keywordAuthor | dopamine | - |
dc.subject.keywordAuthor | dopamine receptor | - |
dc.subject.keywordAuthor | lacrimal gland | - |
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