Protection of the neurovascular unit from calcium-related ischemic injury by linalyl acetate
- Authors
- Hsieh, Yu Shan; Shin, You Kyoung; Seol, Geun Hee
- Issue Date
- 3월-2021
- Publisher
- WOLTERS KLUWER MEDKNOW PUBLICATIONS
- Keywords
- Calcium-related ischemic injury; linalyl acetate; neurovascular unit
- Citation
- CHINESE JOURNAL OF PHYSIOLOGY, v.64, no.2, pp.88 - 96
- Indexed
- SCIE
SCOPUS
- Journal Title
- CHINESE JOURNAL OF PHYSIOLOGY
- Volume
- 64
- Number
- 2
- Start Page
- 88
- End Page
- 96
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/128464
- DOI
- 10.4103/cjp.cjp_94_20
- ISSN
- 0304-4920
- Abstract
- Calcium-related ischemic injury (CRII) can damage cells of the neurovascular unit (NVU). Here, we investigate the protective effects of linalyl acetate (LA) against CRII-induced NVU damage and evaluate the underlying mechanisms. The protective effects of LA in cell lines representative of NVU components (BEND, SH-SY5Y, BV2, and U373 cells) were evaluated following exposure to oxygen-glucose deprivation/reoxygenation alone (OGD/R-only) or OGD/R in the presence of 5 mM extracellular calcium ([Ca2+]o) to mimic CRII. LA reversed damage under OGD/R-only conditions by blocking p47phox/NADPH oxidase (NOX) 2 expression, reactive oxygen species (ROS) production, nitric oxide (NO) abnormality, and lactate dehydrogenase (LDH) release only in the BEND cells. However, under CRII-mimicking conditions, LA reversed NO abnormality and matrix metalloproteinase (MMP)-9 activation in the BEND murine brain endothelial cells; inhibited p47phox expression in the human SH-SY5Y neural-like cells; decreased NOX2 expression and ROS generation in the BV2 murine microglial cells; and reduced p47phox expression in the U373 human astrocyte-like cells. Importantly, LA protected against impairment of the neural cells, astrocytes, and microglia, all of which are cellular components of the NVU induced by exposure to CRII-mimicking conditions, by reducing LDH release. We found that LA exerted a protective effect in the BEND cells that may differ from its protective effects in other NVU cell types, following OGD/R-induced damage in the context of elevated [Ca2+]o.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Nursing > Department of Nursing > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.