Highly sensitive and multiplexed one-step RT-qPCR for profiling genes involved in the circadian rhythm using microparticles
DC Field | Value | Language |
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dc.contributor.author | Kim, M.Y. | - |
dc.contributor.author | Jung, S. | - |
dc.contributor.author | Kim, J. | - |
dc.contributor.author | Lee, H.J. | - |
dc.contributor.author | Jeong, S. | - |
dc.contributor.author | Sim, S.J. | - |
dc.contributor.author | Kim, S.K. | - |
dc.date.accessioned | 2021-12-03T05:42:06Z | - |
dc.date.available | 2021-12-03T05:42:06Z | - |
dc.date.created | 2021-08-31 | - |
dc.date.issued | 2021-03-19 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/129053 | - |
dc.description.abstract | Given the growing interest in molecular diagnosis, highly extensive and selective detection of genetic targets from a very limited amount of samples is in high demand. We demonstrated the highly sensitive and multiplexed one-step RT-qPCR platform for RNA analysis using microparticles as individual reactors. Those particles are equipped with a controlled release system of thermo-responsive materials, and are able to capture RNA targets inside. The particle-based assay can successfully quantify multiple target RNAs from only 200 pg of total RNA. The assay can also quantify target RNAs from a single cell with the aid of a pre-concentration process. We carried out 8-plex one-step RT-qPCR using tens of microparticles, which allowed extensive mRNA profiling. The circadian cycles were shown by the multiplex one-step RT-qPCR in human cell and human hair follicles. Reliable 24-plex one-step RT-qPCR was developed using a single operation in a PCR chip without any loss of performance (i.e., selectivity and sensitivity), even from a single hair. Many other disease-related transcripts can be monitored using this versatile platform. It can also be used non–invasively for samples obtained in clinics. © 2021, The Author(s). | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | Nature Research | - |
dc.title | Highly sensitive and multiplexed one-step RT-qPCR for profiling genes involved in the circadian rhythm using microparticles | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, H.J. | - |
dc.contributor.affiliatedAuthor | Sim, S.J. | - |
dc.identifier.doi | 10.1038/s41598-021-85728-y | - |
dc.identifier.scopusid | 2-s2.0-85102676746 | - |
dc.identifier.wosid | 000632048200009 | - |
dc.identifier.bibliographicCitation | Scientific Reports, v.11, no.1 | - |
dc.relation.isPartOf | Scientific Reports | - |
dc.citation.title | Scientific Reports | - |
dc.citation.volume | 11 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
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