Temperature-specific vsiRNA confers RNAi-mediated viral resistance at elevated temperature in Capsicum annuum
- Authors
- Kim, Yunsik; Kim, Young Jin; Paek, Kyung-Hee
- Issue Date
- 24-2월-2021
- Publisher
- OXFORD UNIV PRESS
- Keywords
- Capsicum annuum (pepper); elevated temperatures; R-mediated resistance; RNAi-mediated antiviral resistance; Tobacco mosaic virus pathotype P-0 (TMV-P-0); virus-derived siRNAs (vsiRNAs)
- Citation
- JOURNAL OF EXPERIMENTAL BOTANY, v.72, no.4, pp.1432 - 1448
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF EXPERIMENTAL BOTANY
- Volume
- 72
- Number
- 4
- Start Page
- 1432
- End Page
- 1448
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/129173
- DOI
- 10.1093/jxb/eraa527
- ISSN
- 0022-0957
- Abstract
- Resistance (R) gene-mediated resistance is a robust and efficient antiviral immune system in the plants. Thus, when R-mediated resistance was suppressed at elevated temperatures, resistance towards viruses was expected to be completely collapsed. Nonetheless, the multiplication of Tobacco mosaic virus pathotype P-0 (TMV-P-0) was inhibited, and TMV-P-0 particles were only occasionally present in the systemic leaves of pepper plants (Capsicum annuum). RNAi-mediated RNA silencing is a well-known antiviral immune mechanism. At elevated temperatures, RNAi-mediated antiviral resistance was induced and virus-derived siRNAs (vsiRNAs) were dramatically increased. Through sRNA-sequencing (sRNA-Seq) analysis, we revealed that vsiRNAs derived from TMV-P-0 were greatly increased. Intriguingly, virus-infected plants could select the temperature-specific vsiRNAs for antiviral resistance from the amplified vsiRNAs at elevated temperatures. Pre-application of these temperature-specific vsiRNAs endowed antiviral resistance of the plants. Therefore, plants sustain antiviral resistance by activating RNAi-mediated resistance, based on temperature-specific vsiRNAs at elevated temperatures.
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Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
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