ExoCAS-2: Rapid and Pure Isolation of Exosomes by Anionic Exchange Using Magnetic Beads
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hyunsung | - |
dc.contributor.author | Shin, Sehyun | - |
dc.date.accessioned | 2021-12-05T01:00:15Z | - |
dc.date.available | 2021-12-05T01:00:15Z | - |
dc.date.created | 2021-08-30 | - |
dc.date.issued | 2021-01 | - |
dc.identifier.issn | 2227-9059 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/129488 | - |
dc.description.abstract | Extracellular vesicles (EVs) are considered essential biomarkers in liquid biopsies. Despite intensive efforts aimed at employing EVs in a clinical setting, workable approaches are currently limited owing to the fact that EV-isolation technologies are still in a nascent stage. This study introduces a magnetic bead-based ion exchange platform for isolating EVs called ExoCAS-2 (exosome clustering and scattering). Owing to their negative charge, exosomes can easily adhere to magnetic beads coated with a polycationic polymer. Owing to the features of magnetic beads, exosomes can be easily processed via washing and elution steps and isolated with high purity and yield within 40 min. The present results confirmed the isolation of exosomes through analyses of size distribution, morphology, surface and internal protein markers, and exosomal RNA. Compared with the commercially available methods, the proposed method showed superior performance in terms of key aspects, including operation time, purity, and recovery rate. This highlights the potential of this magnetic bead-based ion exchange platform for isolating exosomes present in blood plasma. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | CHROMATOGRAPHY | - |
dc.title | ExoCAS-2: Rapid and Pure Isolation of Exosomes by Anionic Exchange Using Magnetic Beads | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Sehyun | - |
dc.identifier.doi | 10.3390/biomedicines9010028 | - |
dc.identifier.scopusid | 2-s2.0-85099796698 | - |
dc.identifier.wosid | 000609867100001 | - |
dc.identifier.bibliographicCitation | BIOMEDICINES, v.9, no.1, pp.1 - 12 | - |
dc.relation.isPartOf | BIOMEDICINES | - |
dc.citation.title | BIOMEDICINES | - |
dc.citation.volume | 9 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 12 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | CHROMATOGRAPHY | - |
dc.subject.keywordAuthor | exosome | - |
dc.subject.keywordAuthor | isolation | - |
dc.subject.keywordAuthor | cationic polymer | - |
dc.subject.keywordAuthor | magnetic beads | - |
dc.subject.keywordAuthor | ion exchange | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.