Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Annona muricataL.-Derived Polysaccharides as a Potential Adjuvant to a Dendritic Cell-Based Vaccine in a Thymoma-Bearing Model

Authors
Kim, Woo SikHan, Jeong MooSong, Ha-YeonByun, Eui-HongLim, Seung-TaikByun, Eui-Baek
Issue Date
6월-2020
Publisher
MDPI
Keywords
polysaccharide; Th1; cytotoxic T lymphocyte; multifunctional T cells; dendritic cells; vaccine adjuvant; Annona muricataL
Citation
NUTRIENTS, v.12, no.6
Indexed
SCIE
SCOPUS
Journal Title
NUTRIENTS
Volume
12
Number
6
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/130617
DOI
10.3390/nu12061602
ISSN
2072-6643
Abstract
Dendritic cells (DCs) are powerful antigen-presenting cells that are often used to evaluate adjuvants, particularly for adjuvant selection for various vaccines. Here, polysaccharides (named ALP) isolated from leaves ofAnnona muricataL., which are used in traditional medicine such as for bacterial infections and inflammatory diseases, were evaluated as an adjuvant candidate that can induce anti-tumor activity. We first confirmed the phenotypic (surface molecules, cytokines, antigen uptake, and antigen-presenting ability) and functional alterations (T cell proliferation/activation) of DCs in vitro. We also confirmed the adjuvant effect by evaluating anti-tumor activity and immunity using an ALP-treated DC-immunized mouse model. ALP functionally induced DC maturation by up-regulating the secretion of Th1-polarizing pro-inflammatory cytokines, the expression of surface molecules, and antigen-presenting ability. ALP triggered DC maturation, which is dependent on the activation of the MAPK and NF-kappa B signaling pathways. ALP-activated DCs showed an ample capacity to differentiate naive T cells to Th1 and activated CD8(+)T cells effectively. The systemic administration of DCs that pulse ALP and ovalbumin peptides strongly increased cytotoxic T lymphocyte (CTL) activity (by 9.5% compared to that in the control vaccine groups), the generation of CD107a-producing multifunctional T cells, and Th1-mediated humoral immunity, and caused a significant reduction (increased protection by 29% over that in control vaccine groups) in tumor growth. ALP, which triggers the Th1 and CTL response, provides a basis for a new adjuvant for various vaccines.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biotechnology > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE