Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Glucose-6-phosphate transporter mediates macrophage proliferation and functions by regulating glycolysis and mitochondrial respiration

Authors
Jeon, Eek HyungPark, Tae SubJang, YuyeonHwang, EunmiKim, Sung-JoSong, Ki-DukWeinstein, David A.Lee, Young MokPark, Byung-ChulJun, Hyun Sik
Issue Date
26-3월-2020
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Keywords
Macrophage; Glucose-6-phosphate transporter; Phagocytosis; Antiviral responses; Metabolic reprogramming
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.524, no.1, pp.89 - 95
Indexed
SCIE
SCOPUS
Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume
524
Number
1
Start Page
89
End Page
95
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/130687
DOI
10.1016/j.bbrc.2020.01.043
ISSN
0006-291X
Abstract
Glycogen storage disease type Ib (GSD-Ib), caused by a deficiency in glucose-6-phosphate transporter (G6PT), is characterized by disrupted glucose homeostasis, inflammatory bowel disease, neutropenia, and neutrophil dysfunction. The purpose of this study was to investigate the role of G6PT on macrophage functions and metabolism. Peritoneal macrophages of G6pt(-/-) mice were lower in number and their effector functions including migration, superoxide production, and phagocytosis were impaired. To investigate the underlying mechanisms of macrophage dysfunction, the G6PT gene was mutated in porcine alveolar macrophage 3D4/31 cells using the CRISPR/Cas9 technology. The G6PT-deficient macrophages exhibited significant decline in cell growth, bactericidal activity, and antiviral response. These phenotypes are associated with the impaired glycolysis and mitochondrial oxidative phosphorylation. We therefore propose that the G6PT-mediated metabolism is essential for effector functions of macrophage, the immune deficiencies observed in GSD-Ib extend beyond neutropenia and neutrophil dysfunction, and future therapeutic targets aimed both the neutrophils and macrophages may be necessary. (C) 2020 Elsevier Inc. All rights reserved.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biotechnology and Bioinformatics > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE