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Nanofibrous Electrospun Heart Decellularized Extracellular Matrix-Based Hybrid Scaffold as Wound Dressing for Reducing Scarring in Wound Healing

Authors
Kim, Tae HeeJung, YoungmeeKim, Soo Hyun
Issue Date
5월-2018
Publisher
MARY ANN LIEBERT, INC
Keywords
heart decellularized extracellular matrix; angiogenesis; wound healing; scarless wound dressing
Citation
TISSUE ENGINEERING PART A, v.24, no.9-10, pp.830 - 848
Indexed
SCIE
SCOPUS
Journal Title
TISSUE ENGINEERING PART A
Volume
24
Number
9-10
Start Page
830
End Page
848
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/131793
DOI
10.1089/ten.tea.2017.0318
ISSN
1937-3341
Abstract
Produced through electrospinning, poly(l-lactide-co-caprolactone) (PLCL) membranes, which have a porous structure and are biodegradable, are of interest in various medical fields. The porous-structured electrospun membrane is particularly interesting because of several favorable properties as follows: it exudes fluid from the wound, does not build up under the wound covering, and does not cause wound desiccation. Moreover, extracellular matrix (ECM)-based structures derived by tissue decellularization have application as engineered tissue scaffolds and as supports for cellular regeneration. In particular, heart decellularized ECM (hdECM) has various pro-angiogenic factors that can induce angiogenesis for wound healing. In this regard, a nanofibrous electrospun hdECM-based hybrid scaffold (NEhdHS), which is a PLCL membrane, including hdECM as an active agent, was tested as a wound dressing to assess its fundamental biochemical and physical features in wound healing. Use of NEhdHS with its porous structure and pro-angiogenic factors is expected to provide an effective wound dressing and reduced scarring. We first demonstrate the effectiveness of a proposed decellularization protocol through analysis of dECM components and describe the mechanical properties of the fabricated NEhdHS. Next, we present an in vitro angiogenesis analysis of the NEhdHS, using a coculture system with human dermal fibroblasts and human umbilical vein endothelial cells; the results of which confirm its biocompatibility and show that the NEhdHS can significantly enhance angiogenesis over that obtained from PLCL or gelatin-containing PLCL scaffolds. We also studied the effectiveness of the NEhdHS in vivo. Using a rat excisional wound-splinting model, we show that covering the upper part of the wound with NEhdHS significantly reduces scarring in the wound healing process compared to that with PLCL or gelatin-containing PLCL scaffolds. Based upon its properties, we conclude that the NEhdHS has potential for application in wound dressing.
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Graduate School > KU-KIST Graduate School of Converging Science and Technology > 1. Journal Articles

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