Improving Osteogenesis Activity on BMP-2-Immobilized PCL Fibers Modified by the gamma-Ray Irradiation Technique
- Authors
- Yun, Young-Pil; Lee, Jae Yong; Jeong, Won Jae; Park, Kyeongsoon; Kim, Hak-Jun; Song, Jae-Jun; Kim, Sung Eun; Song, Hae-Ryong
- Issue Date
- 2015
- Publisher
- HINDAWI LTD
- Citation
- BIOMED RESEARCH INTERNATIONAL, v.2015
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOMED RESEARCH INTERNATIONAL
- Volume
- 2015
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/133200
- DOI
- 10.1155/2015/302820
- ISSN
- 2314-6133
- Abstract
- The purpose of this study was to demonstrate the ability of BMP-2-immobilized polycaprolactone (PCL) fibers modified using the gamma-ray irradiation technique to induce the osteogenic differentiation of MG-63 cells. Poly acrylic acid (AAc) was grafted onto the PCL fibers by the gamma-ray irradiation technique. BMP-2 was then subsequently immobilized onto the AAc-PCL fibers (BMP2/AAc-PCL). PCL and surface-modified PCL fibers was characterized by evaluation with a scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle. The biological activity of the PCL and surface-modified PCL fibers were characterized by alkaline phosphatase (ALP) activity, calcium deposition, and them RNA expression of osteocalcin and osteopontin in MG-63 cells. Successfully grafted AAc and PCL fibers with immobilized BMP-2 were confirmed by XPS results. The results of the contact angle showed that BMP-2/AAc-PCL fibers have more hydrophilic properties in comparison to PCL fibers. The ALP activity, calcium deposition, and gene expressions of MG-63 cells grown on BMP-2/AAc-PCL fibers showed greatly induced osteogenic differentiation in comparison to the PCL fibers. In conclusion, these results demonstrated that BMP-2/AAc-PCL fibers have the potential to effectively induce the osteogenic differentiation of MG-63 cells.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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