Corpus Cavernosal Smooth Muscle Relaxation Effect of a Novel AMPK Activator, Beta-Lapachone
DC Field | Value | Language |
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dc.contributor.author | Bae, Jae Hyun | - |
dc.contributor.author | Kim, Jin Wook | - |
dc.contributor.author | Kweon, Gi Ryang | - |
dc.contributor.author | Park, Myoung Gyu | - |
dc.contributor.author | Jeong, Kyeong-Hoon | - |
dc.contributor.author | Kim, Je Jong | - |
dc.contributor.author | Moon, Du Geon | - |
dc.date.accessioned | 2022-01-03T08:40:36Z | - |
dc.date.available | 2022-01-03T08:40:36Z | - |
dc.date.created | 2021-08-30 | - |
dc.date.issued | 2011-08 | - |
dc.identifier.issn | 1743-6095 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/134223 | - |
dc.description.abstract | Introduction. Adenosine monophosphate-activated protein kinase (AMPK) activation is suggested to relax smooth muscle by endothelial nitric oxide synthase (eNOS) phosphorylation. Aim. To assess the mechanism and effect of a novel AMPK activator, beta-lapachone, upon cavernosal smooth muscle relaxation and the therapeutic potential for erectile dysfunction. Methods. Human umbilical vein endothelial cells (HUVECs) were treated with beta-lapachone. The lysates were blotted with specific antibodies for phosphorylated AMPK (p-AMPK) or phosphorylated eNOS (p-eNOS). The membranes were re-blotted for total AMPK total eNOS, or beta-actin. The eNOS activity was measured by the conversion of L-14C-arginine to L-14C-citrulline in HUVECs lysates. In a separated experiment, cavernosal strips from New Zealand white rabbits were harvested for organ bath study and the relaxation effect of beta-lapachone on phenylephrine-induced contracted strips was evaluated and compared with sodium nitroprusside, zaprinast, metformin, and aminoimidazole carboxamide ribonucleotide (AICAR). Methylene blue and L-NAME were used to assess the inhibition of cyclic guanosine monophosphate/nitric oxide pathway. Zinc-protoporphyrin-IX (ZnPP) was also used to investigate the contribution of mevalonate pathway. Main Outcome Measures. The expression of p-AMPK, p-eNOS, AMPK and eNOS induced by beta-lapachone in HUVECs study and the percent relaxation of cavernosal tissue in organ bath study. Results. Beta-lapachone clearly induced AMPK phosphorylation and, as a consequence, eNOS phosphorylation in HUVECs. Beta-lapachone-induced upregulation of eNOS activity was also observed in HUVECs and steadily increased up to 1 hour. In organ bath study, beta-lapachone significantly relaxed the phenylephrine pretreated strips in a dose-dependent manner. This relaxation effect was not totally blocked by methylene blue or L-NAME. After removing endothelium, the relaxation was totally blocked by ZnPP. Conclusions. A novel AMPK activator, beta-lapachone has a strong relaxation effect on precontracted cavernosal smooth muscle strips in the rabbit. And phosphorylation of AMPK and eNOS strongly related to the action of beta-lapachone. Mevalonate pathway also might be considered as a suggestive mechanism. Bae JH, Kim JW, Kweon GR, Park MG, Jeong K-H, Kim JJ, and Moon DG. Corpus cavernosal smooth muscle relaxation effect of a novel AMPK activator, beta-lapachone. J Sex Med 2011;8:2205-2214. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | NITRIC-OXIDE SYNTHESIS | - |
dc.subject | PROTEIN-KINASE | - |
dc.subject | CARBON-MONOXIDE | - |
dc.subject | ENDOTHELIAL-CELLS | - |
dc.subject | SKELETAL-MUSCLE | - |
dc.subject | CANCER-CELLS | - |
dc.subject | UNDERLYING MECHANISMS | - |
dc.subject | METABOLIC SYNDROME | - |
dc.subject | HEME OXYGENASE | - |
dc.subject | RAT-HEART | - |
dc.title | Corpus Cavernosal Smooth Muscle Relaxation Effect of a Novel AMPK Activator, Beta-Lapachone | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Bae, Jae Hyun | - |
dc.contributor.affiliatedAuthor | Kim, Jin Wook | - |
dc.contributor.affiliatedAuthor | Kim, Je Jong | - |
dc.contributor.affiliatedAuthor | Moon, Du Geon | - |
dc.identifier.doi | 10.1111/j.1743-6109.2010.01809.x | - |
dc.identifier.scopusid | 2-s2.0-79960829688 | - |
dc.identifier.wosid | 000293185500010 | - |
dc.identifier.bibliographicCitation | JOURNAL OF SEXUAL MEDICINE, v.8, no.8, pp.2205 - 2214 | - |
dc.relation.isPartOf | JOURNAL OF SEXUAL MEDICINE | - |
dc.citation.title | JOURNAL OF SEXUAL MEDICINE | - |
dc.citation.volume | 8 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 2205 | - |
dc.citation.endPage | 2214 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Urology & Nephrology | - |
dc.relation.journalWebOfScienceCategory | Urology & Nephrology | - |
dc.subject.keywordPlus | NITRIC-OXIDE SYNTHESIS | - |
dc.subject.keywordPlus | PROTEIN-KINASE | - |
dc.subject.keywordPlus | CARBON-MONOXIDE | - |
dc.subject.keywordPlus | SKELETAL-MUSCLE | - |
dc.subject.keywordPlus | CANCER-CELLS | - |
dc.subject.keywordPlus | UNDERLYING MECHANISMS | - |
dc.subject.keywordPlus | METABOLIC SYNDROME | - |
dc.subject.keywordPlus | HEME OXYGENASE | - |
dc.subject.keywordPlus | RAT-HEART | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordAuthor | AMPK Activator | - |
dc.subject.keywordAuthor | Beta-Lapachone | - |
dc.subject.keywordAuthor | Penile Erection | - |
dc.subject.keywordAuthor | eNOS | - |
dc.subject.keywordAuthor | Corpus Cavernosum | - |
dc.subject.keywordAuthor | Smooth Muscle Relaxation | - |
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