Molecular Analysis of a Prolonged Spread of Klebsiella pneumoniae Co-producing DHA-1 and SHV-12 beta-Lactamases
DC Field | Value | Language |
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dc.contributor.author | Yoon, Young Kyung | - |
dc.contributor.author | Cheong, Hye Won | - |
dc.contributor.author | Pai, Hyunjoo | - |
dc.contributor.author | Roh, Kyoung Ho | - |
dc.contributor.author | Kim, Jeong Yeon | - |
dc.contributor.author | Park, Dae Won | - |
dc.contributor.author | Sohn, Jang Wook | - |
dc.contributor.author | Lee, Seung Eun | - |
dc.contributor.author | Chun, Byung Chul | - |
dc.contributor.author | Sim, Hee Sun | - |
dc.contributor.author | Kim, Min Ja | - |
dc.date.accessioned | 2022-01-03T09:40:53Z | - |
dc.date.available | 2022-01-03T09:40:53Z | - |
dc.date.created | 2021-08-30 | - |
dc.date.issued | 2011-06 | - |
dc.identifier.issn | 1225-8873 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/134229 | - |
dc.description.abstract | The study investigated molecular mechanisms for prolonged nosocomial spread of multidrug-resistant Klebsiella pneumoniae co-producing plasmid-mediated AmpC beta-lactamase DHA-1 and extended-spectrum beta-lactamase SHV-12. Forty-eight clinical isolates of K pneumonia, resistant to the extended-spectrum cephalosporins, were collected in a 750-bed university hospital over a year. The isolates were characterized for PCR-based beta-lactamase genotypes, isoelectric focusing and pulsed-field gel electrophoresis (PFGE) profiles. Resistance transfer was performed by plasmid conjugation and confirmed by a duplex-PCR and Southern hybridization. On beta-lactamase typing, the strains producing only the DHA-1 enzyme (n=17) or co-producing DHA-1 and SHV-12 enzymes (n=15) were predominant. Judging from a one year-distribution of PFGE profiles, the co-producer was spread primarily with single clonal expansion of the PFGE-type A with subtypes (n=14), whereas the strains producing only DHA-1 enzyme were spread simultaneously with the PFGE-type A (n=11) and other PFGE types (n=6). Transconjugants of the co-producers were confirmed to harbor either both bla(DHA-1) and bla(SHV-12) or only the bla(DHA-1). In conclusion, this study indicated that the persistent nosocomial spread of multidrug-resistant K. pneumoniae strains was primarily associated with expansion of a clone harboring both the bla(DHA-1) and bla(SHV-12) or the bla(DHA-1) only, and to a lesser extent with the horizontal transfer of the resistant plasmids. Our observations have clinical implication for the control and prevention of nosocomial dissemination of multidrug-resistant K. pneumoniae strains. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MICROBIOLOGICAL SOCIETY KOREA | - |
dc.subject | PLASMID-MEDIATED AMPC | - |
dc.subject | BLOOD-STREAM INFECTIONS | - |
dc.subject | INTENSIVE-CARE-UNIT | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | CTX-M | - |
dc.subject | CLINICAL ISOLATE | - |
dc.subject | KOREA | - |
dc.subject | DISSEMINATION | - |
dc.subject | PREVALENCE | - |
dc.subject | EMERGENCE | - |
dc.title | Molecular Analysis of a Prolonged Spread of Klebsiella pneumoniae Co-producing DHA-1 and SHV-12 beta-Lactamases | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yoon, Young Kyung | - |
dc.contributor.affiliatedAuthor | Roh, Kyoung Ho | - |
dc.contributor.affiliatedAuthor | Park, Dae Won | - |
dc.contributor.affiliatedAuthor | Sohn, Jang Wook | - |
dc.contributor.affiliatedAuthor | Chun, Byung Chul | - |
dc.contributor.affiliatedAuthor | Kim, Min Ja | - |
dc.identifier.doi | 10.1007/s12275-011-0491-9 | - |
dc.identifier.scopusid | 2-s2.0-79959687312 | - |
dc.identifier.wosid | 000292249100005 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY, v.49, no.3, pp.363 - 368 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY | - |
dc.citation.volume | 49 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 363 | - |
dc.citation.endPage | 368 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001566686 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | PLASMID-MEDIATED AMPC | - |
dc.subject.keywordPlus | BLOOD-STREAM INFECTIONS | - |
dc.subject.keywordPlus | INTENSIVE-CARE-UNIT | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | CTX-M | - |
dc.subject.keywordPlus | CLINICAL ISOLATE | - |
dc.subject.keywordPlus | KOREA | - |
dc.subject.keywordPlus | DISSEMINATION | - |
dc.subject.keywordPlus | PREVALENCE | - |
dc.subject.keywordPlus | EMERGENCE | - |
dc.subject.keywordAuthor | Klebsiella pneumoniae | - |
dc.subject.keywordAuthor | spread | - |
dc.subject.keywordAuthor | plasmid | - |
dc.subject.keywordAuthor | DHA-1 | - |
dc.subject.keywordAuthor | SHV-12 | - |
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