Silencing of the GnRH Type 1 Receptor Blocks the Antiproliferative Effect of the GnRH Agonist, Leuprolide, on the Androgen-independent Prostate Cancer Cell Line DU145
- Authors
- Ko, Y. H.; Ha, Y. R.; Kim, J. W.; Kang, S. G.; Jang, H. A.; Kang, S. H.; Park, H. S.; Cheon, J.
- Issue Date
- 5월-2011
- Publisher
- FIELD HOUSE PUBLISHING LLP
- Keywords
- LEUPROLIDE; GONADOTROPIN-RELEASING HORMONE (GNRH) AGONIST; PROSTATE CANCER; HORMONE THERAPY; DU145 CELLS; CELL PROLIFERATION; GNRH TYPE 1 RECEPTOR
- Citation
- JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, v.39, no.3, pp.729 - 739
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
- Volume
- 39
- Number
- 3
- Start Page
- 729
- End Page
- 739
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/134235
- DOI
- 10.1177/147323001103900304
- ISSN
- 0300-0605
- Abstract
- This study investigated the mechanism of action of a gonadotropin-releasing hormone (GnRH) agonist, leuprolide, on proliferation of the hormone-refractory prostate cancer cell line DU145, transfected with short hairpin RNA (shRNA), to reduce expression of the GNRHR1 gene (which encodes the GnRH type 1 receptor). DU145 cell proliferation in the presence of leuprolide (10(-9) and 10(-7) M) or control medium was measured before and after GnRHR1 knockdown. Reverse transcription-polymerase chain reaction and Western blot analysis were used to measure the degree of GNRHR1 silencing. DU145 cells treated with leuprolide (10(-9) and 10(-7) M) showed significant growth inhibition compared with control-treated DU145 cells. Transfection with GNRHR1-shRNA significantly decreased GNRHR1 expression at 48 h. DU145 cells transfected with silencing GNRHR1-shRNA showed normal growth patterns; however, there was no significant inhibition of proliferation of DU145 cells transfected with GNRHR/-shRNA compared with cells transfected with control-shRNA in response to leuprolide. These data demonstrated that the antiproliferative effect of leuprolide was mediated by the GnRHR1.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
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