Ion beam fabrication of an antifouling Pluronic F-108 thin film-based microwell bioplatform for highly resolved cell microarrays
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hwang, In-Tae | - |
dc.contributor.author | Yoo, Young-Do | - |
dc.contributor.author | Jung, Chan-Hee | - |
dc.contributor.author | Choi, Jae-Hak | - |
dc.date.accessioned | 2022-02-10T19:40:23Z | - |
dc.date.available | 2022-02-10T19:40:23Z | - |
dc.date.created | 2022-01-19 | - |
dc.date.issued | 2022-01-30 | - |
dc.identifier.issn | 0169-4332 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/135250 | - |
dc.description.abstract | Cell microarray bioplatform have been widely developed for fundamental and advanced biomedical research. However, it is still difficult to achieve site-specific adhesion due to the weak cell repellency of the background regions. Here, a microwell bioplatform with a strong cell-repellent background was newly developed by a sequential ion beam irradiation-induced anchoring and re-irradiation-induced erosion microstructuring of PF108 thin film on a tissue culture polystyrene (TCPS) substrate. The newly developed bioplatform possesses outstanding optical transparency and well-defined microwells in a variety of sizes and shapes. Its microwells also show cell-adhesiveness (as much as that on the TCPS) with excellent cell-repellency (no cell growth) on the anchored PF-108 film background. As a result, the live-cell culturing of different strains on the developed bioplatform produced two highly resolved cell microarrays (possessing the diverse sizes and shapes corresponding to those of the bioplatform) with a non-specific adhesion-free background. Moreover, different whole-slide GFP and dsRED protein expressions without a non-specific binding background were successfully achieved in the cell microarray. Noticeably, our finding demonstrates that this toxic chemical-free and biocompatible strategy using ion beam-induced solid-state microstructuring provides a non-specific binding-free microwell bioplatform for use in a highly resolved cell microarray for the practical gene expression assay. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.subject | STAINLESS-STEEL SURFACES | - |
dc.subject | PLASMA DEPOSITION | - |
dc.subject | HYPERBRANCHED POLYGLYCEROL | - |
dc.subject | REVERSE TRANSFECTION | - |
dc.subject | DIMETHYL-ETHER | - |
dc.subject | POLYMER | - |
dc.subject | SUBSTRATE | - |
dc.subject | ADHESION | - |
dc.subject | GLASS | - |
dc.subject | PEO | - |
dc.title | Ion beam fabrication of an antifouling Pluronic F-108 thin film-based microwell bioplatform for highly resolved cell microarrays | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yoo, Young-Do | - |
dc.identifier.doi | 10.1016/j.apsusc.2021.151551 | - |
dc.identifier.scopusid | 2-s2.0-85117363029 | - |
dc.identifier.wosid | 000723693600005 | - |
dc.identifier.bibliographicCitation | APPLIED SURFACE SCIENCE, v.573 | - |
dc.relation.isPartOf | APPLIED SURFACE SCIENCE | - |
dc.citation.title | APPLIED SURFACE SCIENCE | - |
dc.citation.volume | 573 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Coatings & Films | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.relation.journalWebOfScienceCategory | Physics, Condensed Matter | - |
dc.subject.keywordPlus | STAINLESS-STEEL SURFACES | - |
dc.subject.keywordPlus | PLASMA DEPOSITION | - |
dc.subject.keywordPlus | HYPERBRANCHED POLYGLYCEROL | - |
dc.subject.keywordPlus | REVERSE TRANSFECTION | - |
dc.subject.keywordPlus | DIMETHYL-ETHER | - |
dc.subject.keywordPlus | POLYMER | - |
dc.subject.keywordPlus | SUBSTRATE | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | GLASS | - |
dc.subject.keywordPlus | PEO | - |
dc.subject.keywordAuthor | Ion irradiation | - |
dc.subject.keywordAuthor | Solid-state chemistry | - |
dc.subject.keywordAuthor | Antifouling polymer | - |
dc.subject.keywordAuthor | Microstructure | - |
dc.subject.keywordAuthor | Cell microarray | - |
dc.subject.keywordAuthor | Gene transfection | - |
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