Ultrahigh-b diffusion-weighted imaging for quantitative evaluation of myelination in shiverer mouse spinal cord
DC Field | Value | Language |
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dc.contributor.author | Jeong, Kyle E. | - |
dc.contributor.author | Lee, Sophie YouJung | - |
dc.contributor.author | Yeom, Suk-Keu | - |
dc.contributor.author | Carlson, Noel | - |
dc.contributor.author | Shah, Lubdha M. | - |
dc.contributor.author | Rose, John | - |
dc.contributor.author | Jeong, Eun-Kee | - |
dc.date.accessioned | 2022-02-11T09:40:20Z | - |
dc.date.available | 2022-02-11T09:40:20Z | - |
dc.date.created | 2022-02-07 | - |
dc.date.issued | 2022-01 | - |
dc.identifier.issn | 0740-3194 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/135320 | - |
dc.description.abstract | Purpose To perform a quantitative evaluation of myelination on WT and myelin-deficient (shiverer) mouse spinal cords using ultrahigh-b diffusion-weighted imaging (UHb-DWI). Methods UHb-DWI of ex vivo on spinal cord specimens of two shiverer (C3HeB/FeJ-shiverer, homozygous genotype for MbP(shi)) and six WT (Black Six, C3HeB/FeJ) mice were acquired using 3D multishot diffusion-weighted stimulated-echo EPI, a homemade RF coil, and a small-bore 7T MRI system. Imaging was performed in transaxial plane with 75 x 75 mu m(2) in-plane resolution, 1-mm-slice thickness, and radial DWI using b(max) = 42,890 s/mm(2). Histological evaluation was performed on upper thoracic sections using optical and transmission electron microscopy. Numerical Monte Carlo simulations (MCSs) of water diffusion were performed to facilitate interpretation of UHb-DWI signal-b curves. Results The white matter ultrahigh-b radial DWI (UHb-rDWI) signal-b curves of WT mouse cords behaved biexponentially with high-b diffusion coefficient D-H < 0.020 x 10(-3) mm(2)/s. However, as expected with less myelination, the signal-b of shiverer mouse cords behaved monoexponentially with significantly greater D-H = 0.162 x 10(-3), 0.142 x 10(-3), and 0.164 x 10(-3) mm(2)/s at anterodorsal, posterodorsal, and lateral columns, respectively. The axial DWI signals of all mouse cords behaved monoexponentially with D = (0.718-1.124) x 10(-3) mm(2)/s. MCS suggests that these elevated D-H are mainly induced by increased water exchange at the myelin sheath. Microscopic results were consistent with the UHb-rDWI findings. Conclusion UHb-DWI provides quantitative differences in myelination of spinal cords from myelin-deficit shiverer and WT mice. UHb-DWI may become a powerful tool to evaluate myelination in demyelinating disease models that may translate to human diseases, including multiple sclerosis. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | MAGNETIZATION-TRANSFER MRI | - |
dc.subject | MULTIPLE-SCLEROSIS | - |
dc.subject | IN-VIVO | - |
dc.subject | OPTIC-NERVE | - |
dc.subject | WATER DIFFUSION | - |
dc.subject | WHITE-MATTER | - |
dc.subject | AXON | - |
dc.subject | DEMYELINATION | - |
dc.subject | QUANTIFICATION | - |
dc.subject | PATHOLOGY | - |
dc.title | Ultrahigh-b diffusion-weighted imaging for quantitative evaluation of myelination in shiverer mouse spinal cord | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yeom, Suk-Keu | - |
dc.identifier.doi | 10.1002/mrm.28978 | - |
dc.identifier.scopusid | 2-s2.0-85113149274 | - |
dc.identifier.wosid | 000686943600001 | - |
dc.identifier.bibliographicCitation | MAGNETIC RESONANCE IN MEDICINE, v.87, no.1, pp.179 - 192 | - |
dc.relation.isPartOf | MAGNETIC RESONANCE IN MEDICINE | - |
dc.citation.title | MAGNETIC RESONANCE IN MEDICINE | - |
dc.citation.volume | 87 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 179 | - |
dc.citation.endPage | 192 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.relation.journalWebOfScienceCategory | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.subject.keywordPlus | MAGNETIZATION-TRANSFER MRI | - |
dc.subject.keywordPlus | MULTIPLE-SCLEROSIS | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | OPTIC-NERVE | - |
dc.subject.keywordPlus | WATER DIFFUSION | - |
dc.subject.keywordPlus | WHITE-MATTER | - |
dc.subject.keywordPlus | AXON | - |
dc.subject.keywordPlus | DEMYELINATION | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | PATHOLOGY | - |
dc.subject.keywordAuthor | diffusion kurtosis imaging | - |
dc.subject.keywordAuthor | diffusion-weighted imaging | - |
dc.subject.keywordAuthor | electron microscopy | - |
dc.subject.keywordAuthor | multiple sclerosis | - |
dc.subject.keywordAuthor | optical microscopy | - |
dc.subject.keywordAuthor | shiverer mice | - |
dc.subject.keywordAuthor | spinal cord | - |
dc.subject.keywordAuthor | Ultrahigh-b diffusion-weighted imaging | - |
dc.subject.keywordAuthor | white matter | - |
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