Diagnostic usefulness of subgenomic RNA detection of viable SARS-CoV-2 in patients with COVID-19
DC Field | Value | Language |
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dc.contributor.author | Kim, Ji Yeun | - |
dc.contributor.author | Bae, Joon-Yong | - |
dc.contributor.author | Bae, Seongman | - |
dc.contributor.author | Cha, Hye Hee | - |
dc.contributor.author | Kwon, Ji-Soo | - |
dc.contributor.author | Suh, Mi Hyun | - |
dc.contributor.author | Lee, Hyun Jung | - |
dc.contributor.author | Jung, Jiwon | - |
dc.contributor.author | Kim, Min Jae | - |
dc.contributor.author | Cui, Chunguang | - |
dc.contributor.author | Park, Heedo | - |
dc.contributor.author | Lee, Jungmin | - |
dc.contributor.author | Park, Man-Seong | - |
dc.contributor.author | Kim, Sung-Han | - |
dc.date.accessioned | 2022-02-11T11:40:19Z | - |
dc.date.available | 2022-02-11T11:40:19Z | - |
dc.date.created | 2022-01-19 | - |
dc.date.issued | 2022-01 | - |
dc.identifier.issn | 1198-743X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/135330 | - |
dc.description.abstract | Objectives: The development of a rapid diagnostic test for viable SARS-CoV-2 is important for infection control. Real-time RT-PCR assays detect non-viable virus, and cell culture differentiates viable virus but it takes several weeks and is labour-intensive. Subgenomic RNAs may reflect replication-competent virus. We therefore evaluated the usefulness of subgenomic RNAs for diagnosing viable SARS-CoV-2 in patients with COVID-19. Methods: Patients with various severities of confirmed COVID-19 were enrolled at a tertiary hospital between February and December 2020. RT-PCR assay results for genomic and subgenomic RNA of SARSCoV-2 from nasopharyngeal swab, sputum and saliva specimens were compared with cell culture results. Results: A total 189 specimens from 20 COVID-19 patients were tested in genomic and subgenomic PCR assays and cultured on Vero cells. Of these 189 samples, 62 (33%) gave positive culture results, 93 (49%) negative results and the remaining 34 (18%) indeterminate results. Compared with cell culture results, the sensitivities of genomic RNA and subgenomic RNA of the N and S genes were comparable at 100%, but the specificity of subgenomic RNA (N, 65% and S, 68%) was higher than that of genomic RNA (N, 23% and S, 17%, p < 0.001). The mean durations of positive culture and subgenomic RNA were 11.39 +/- 10.34 and 13.75 +/- 11.22 days after symptom onset (p 0.437), respectively, while that of genomic RNA was 22.85 +/- 11.83 days after symptom onset (p < 0.001). Discussion: Our comparison of subgenomic RNA detection with symptom duration and SARS-CoV-2 culture positivity provides a significant advancement on the transmissibility-based approach beyond the detection of SARS-CoV-2 genomic RNA, and warrants further studies on the development of better diagnostic strategy. Ji Yeun Kim, Clin Microbiol Infect 2022;28:101 (c) 2021 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Diagnostic usefulness of subgenomic RNA detection of viable SARS-CoV-2 in patients with COVID-19 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Man-Seong | - |
dc.identifier.doi | 10.1016/j.cmi.2021.08.009 | - |
dc.identifier.scopusid | 2-s2.0-85114181525 | - |
dc.identifier.wosid | 000735207100016 | - |
dc.identifier.bibliographicCitation | CLINICAL MICROBIOLOGY AND INFECTION, v.28, no.1, pp.101 - 106 | - |
dc.relation.isPartOf | CLINICAL MICROBIOLOGY AND INFECTION | - |
dc.citation.title | CLINICAL MICROBIOLOGY AND INFECTION | - |
dc.citation.volume | 28 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 101 | - |
dc.citation.endPage | 106 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Infectious Diseases | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Infectious Diseases | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordAuthor | COVID-19 | - |
dc.subject.keywordAuthor | Infective viral shedding | - |
dc.subject.keywordAuthor | SARS CoV-2 | - |
dc.subject.keywordAuthor | Subgenomic RNA | - |
dc.subject.keywordAuthor | Virus culture | - |
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