Detection of Microsatellite Instability in Colorectal Cancer Patients With a Plasma-Based Real-Time PCR Analysis
DC Field | Value | Language |
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dc.contributor.author | Kim, Namjoo | - |
dc.contributor.author | Kim, Sung Min | - |
dc.contributor.author | Lee, Beom Jae | - |
dc.contributor.author | Choi, Byung il | - |
dc.contributor.author | Yoon, Hee Sook | - |
dc.contributor.author | Kang, Sang Hee | - |
dc.contributor.author | Kim, Seung Han | - |
dc.contributor.author | Joo, Moon Kyung | - |
dc.contributor.author | Park, Jong-Jae | - |
dc.contributor.author | Kim, Chungyeul | - |
dc.date.accessioned | 2022-02-12T07:41:11Z | - |
dc.date.available | 2022-02-12T07:41:11Z | - |
dc.date.created | 2022-01-19 | - |
dc.date.issued | 2021-12-08 | - |
dc.identifier.issn | 1663-9812 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/135457 | - |
dc.description.abstract | A microsatellite instability (MSI) test is crucial for screening for HNPCC (Hereditary nonpolyposis colorectal cancer; Lynch syndrome) and optimization of colorectal cancer (CRC) treatment. Mismatch repair (MMR) deficiency is a predictor for good response of immune checkpoint inhibitors in various malignancies. In this study, we evaluated the results of a newly developed plasma-based real-time PCR kit for the detection of MSI in CRC patients. We assessed a peptide nucleotide acid (PNA) probe-mediated real-time PCR test (U-TOP MSI Detection Kit Plus) that determines MSI status by using amplicon melting analysis of five markers (NR21, NR24, NR27, BAT25, and BAT26) from plasma. Eighty-four CRC patients (46 dMMR and 38 pMMR) with colorectal cancer were analyzed. The concordance rate of MSI status assessment between the plasma kit and IHC was 63.0% in dMMR patients (29/46), but in the pMMR evaluation, a 100% (38/38) concordance rate was observed. In the evaluation of the performance of a custom tissue U-TOP MSI Detection Kit and plasma kit in 28 patients, sensitivity, specificity, PPV (positive predictive value) and NPV (negative predictive value) of plasma kit were 68.4, 100, 100, and 44.4%, respectively, with the tissue U-TOP MSI Detection Kit. Our results demonstrate the feasibility of a non-invasive and rapid plasma-based real-time PCR kit (U-TOP MSI Detection Kit Plus) for the detection of MSI in colorectal cancer. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | FRONTIERS MEDIA SA | - |
dc.subject | CELL-FREE DNA | - |
dc.subject | MISMATCH-REPAIR | - |
dc.subject | BETHESDA GUIDELINES | - |
dc.subject | COLON-CANCER | - |
dc.subject | METHYLATION | - |
dc.subject | VALIDATION | - |
dc.subject | TUMORS | - |
dc.subject | SERUM | - |
dc.title | Detection of Microsatellite Instability in Colorectal Cancer Patients With a Plasma-Based Real-Time PCR Analysis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Beom Jae | - |
dc.identifier.doi | 10.3389/fphar.2021.758830 | - |
dc.identifier.scopusid | 2-s2.0-85121616164 | - |
dc.identifier.wosid | 000732795700001 | - |
dc.identifier.bibliographicCitation | FRONTIERS IN PHARMACOLOGY, v.12 | - |
dc.relation.isPartOf | FRONTIERS IN PHARMACOLOGY | - |
dc.citation.title | FRONTIERS IN PHARMACOLOGY | - |
dc.citation.volume | 12 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | BETHESDA GUIDELINES | - |
dc.subject.keywordPlus | CELL-FREE DNA | - |
dc.subject.keywordPlus | COLON-CANCER | - |
dc.subject.keywordPlus | METHYLATION | - |
dc.subject.keywordPlus | MISMATCH-REPAIR | - |
dc.subject.keywordPlus | SERUM | - |
dc.subject.keywordPlus | TUMORS | - |
dc.subject.keywordPlus | VALIDATION | - |
dc.subject.keywordAuthor | colorectal cancer | - |
dc.subject.keywordAuthor | liquid biopsy | - |
dc.subject.keywordAuthor | microsatellite instability | - |
dc.subject.keywordAuthor | mismatch repair | - |
dc.subject.keywordAuthor | real-time PCR | - |
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