Contributory Role of BLT2 in the Production of Proinflammatory Cytokines in Cecal Ligation and Puncture-Induced Sepsis
- Authors
- Park, Donghwan; Ro, MyungJa; Lee, A-Jin; Kwak, Dong-Wook; Chung, Yunro; Kim, Jae-Hong
- Issue Date
- Dec-2021
- Publisher
- KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
- Keywords
- BLT2; cecal ligation and puncture; cytokines; leukotrienes; sepsis
- Citation
- MOLECULES AND CELLS, v.44, no.12, pp.893 - 899
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- MOLECULES AND CELLS
- Volume
- 44
- Number
- 12
- Start Page
- 893
- End Page
- 899
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/135517
- DOI
- 10.14348/molcells.2021.0159
- ISSN
- 1016-8478
- Abstract
- BLT2 is a low-affinity receptor for leukotriene B-4, a potent lipid mediator of inflammation generated from arachidonic acid via the 5-lipoxygenase pathway. The aim of this study was to investigate whether BLT2 plays any role in sepsis, a systemic inflammatory response syndrome caused by infection. A murine model of cecal ligation and puncture (CLP)-induced sepsis was used to evaluate the role of BLT2 in septic inflammation. In the present study, we observed that the levels of ligands for BLT2 (LTB4 [leukotriene B-4 ] and 12(S)-HETE [12(S)-hydroxyeicosatetraenoic acid]) were significantly increased in the peritoneal lavage fluid and serum from mice with CLP-induced sepsis. We also observed that the levels of BLT2 as well as 5-lipoxygenase (5-LO) and 12-LO, which are synthesizing enzymes for LTB 4 and 12(S)-HETE, were significantly increased in lung and liver tissues in the CLP mouse model. Blockade of BLT2 markedly suppressed the production of sepsis-associated cytokines (IL-6 [interieukin-6], TNF-alpha [tumor necrosis factor alpha], and IL-1 beta [interleukin-1 beta] as well as IL-17 [interleukin-17]) and alleviated lung inflammation in the CLP group. Taken together, our results suggest that BLT2 cascade contributes to lung inflammation in CLP-induced sepsis by mediating the production of inflammatory cytokines. These findings suggest that BLT2 may be a potential therapeutic target for sepsis patients.
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Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
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