Development of a bifunctional BODIPY probe for mitochondria imaging and in situ photo-crosslinking in live cell
DC Field | Value | Language |
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dc.contributor.author | Murale, Dhiraj P. | - |
dc.contributor.author | Haque, Md Mamunul | - |
dc.contributor.author | Hong, Seong Cheol | - |
dc.contributor.author | Jang, Se-young | - |
dc.contributor.author | Lee, Jung Hoon | - |
dc.contributor.author | An, Seo Jeong | - |
dc.contributor.author | Lee, Jun-Seok | - |
dc.date.accessioned | 2022-02-13T06:40:34Z | - |
dc.date.available | 2022-02-13T06:40:34Z | - |
dc.date.created | 2022-02-09 | - |
dc.date.issued | 2021-12 | - |
dc.identifier.issn | 0143-7208 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/135591 | - |
dc.description.abstract | Mitochondria are eukaryotic sub-cellular organelle that play critical roles in energy generation for life. As the morphology of the mitochondria is dramatically versatile, there is a great demand for easy-to-use tools for mitochondria imaging. Until today, many organic probes are known to target mitochondria in live-cell condi-tions, but many of them are suffered from continuous leaking out from mitochondria. To overcome this chal-lenge, we developed a bifunctional BODIPY probe that selectively engaged mitochondria and spontaneously produced the chemical crosslinking by photo-activation. To systematically optimize the dual functions, we synthesized five BODIPY fluorophores and investigated their photophysical and photo-crosslinking properties by comparing series of photo-crosslinking functional groups and their modification positions in BODIPY fluo-rophore. Finally, we discovered not only pcBDaza-Mito selectively stains mitochondria in live-cell condition, but also it exhibited 2.3 folds increase for photo-crosslinking efficiency to mitochondria proteins. These results present that the bifunctional BODIPY probe has great merit for robust mitochondria imaging in a live cell. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | PHOTOAFFINITY LINKERS | - |
dc.subject | FLUORESCENT-PROBES | - |
dc.subject | TARGET | - |
dc.subject | PROTEINS | - |
dc.subject | LIBRARY | - |
dc.subject | DYES | - |
dc.title | Development of a bifunctional BODIPY probe for mitochondria imaging and in situ photo-crosslinking in live cell | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Jun-Seok | - |
dc.identifier.doi | 10.1016/j.dyepig.2021.109830 | - |
dc.identifier.scopusid | 2-s2.0-85115892322 | - |
dc.identifier.wosid | 000704429000007 | - |
dc.identifier.bibliographicCitation | DYES AND PIGMENTS, v.196 | - |
dc.relation.isPartOf | DYES AND PIGMENTS | - |
dc.citation.title | DYES AND PIGMENTS | - |
dc.citation.volume | 196 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Textiles | - |
dc.subject.keywordPlus | DYES | - |
dc.subject.keywordPlus | FLUORESCENT-PROBES | - |
dc.subject.keywordPlus | LIBRARY | - |
dc.subject.keywordPlus | PHOTOAFFINITY LINKERS | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | TARGET | - |
dc.subject.keywordAuthor | Bifunctional probe | - |
dc.subject.keywordAuthor | Fluorescent probe | - |
dc.subject.keywordAuthor | Live cell sub-organelle imaging | - |
dc.subject.keywordAuthor | Mitochondria | - |
dc.subject.keywordAuthor | Photo-crosslinking probe | - |
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