Generation of Induced Nephron Progenitor-like Cells from Human Urine-Derived Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gao, Wei-Wei | - |
dc.contributor.author | Zheng, Jie | - |
dc.contributor.author | Yun, Wonjin | - |
dc.contributor.author | Kang, Phil-Jun | - |
dc.contributor.author | Park, Gyuman | - |
dc.contributor.author | Song, Gwonhwa | - |
dc.contributor.author | Kim, In-Yong | - |
dc.contributor.author | You, Seungkwon | - |
dc.date.accessioned | 2022-02-13T18:41:18Z | - |
dc.date.available | 2022-02-13T18:41:18Z | - |
dc.date.created | 2022-01-19 | - |
dc.date.issued | 2021-12 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/135654 | - |
dc.description.abstract | Background: Regenerative medicine strategies employing nephron progenitor cells (NPCs) are a viable approach that is worthy of substantial consideration as a promising cell source for kidney diseases. However, the generation of induced nephron progenitor-like cells (iNPCs) from human somatic cells remains a major challenge. Here, we describe a novel method for generating NPCs from human urine-derived cells (UCs) that can undergo long-term expansion in a serum-free condition. Results: Here, we generated iNPCs from human urine-derived cells by forced expression of the transcription factors OCT4, SOX2, KLF4, c-MYC, and SLUG, followed by exposure to a cocktail of defined small molecules. These iNPCs resembled human embryonic stem cell-derived NPCs in terms of their morphology, biological characteristics, differentiation potential, and global gene expression and underwent a long-term expansion in serum-free conditions. Conclusion: This study demonstrates that human iNPCs can be readily generated and expanded, which will facilitate their broad applicability in a rapid, efficient, and patient-specific manner, particularly holding the potential as a transplantable cell source for patients with kidney disease. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | PLURIPOTENT STEM-CELLS | - |
dc.subject | KIDNEY EPITHELIAL-CELLS | - |
dc.subject | MOUSE FIBROBLASTS | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | CARDIOMYOCYTES | - |
dc.subject | DISSOCIATION | - |
dc.subject | MESENCHYME | - |
dc.subject | CONVERSION | - |
dc.subject | PODOCYTES | - |
dc.title | Generation of Induced Nephron Progenitor-like Cells from Human Urine-Derived Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Gwonhwa | - |
dc.identifier.doi | 10.3390/ijms222413449 | - |
dc.identifier.scopusid | 2-s2.0-85121050033 | - |
dc.identifier.wosid | 000738622900001 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.22, no.24 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 22 | - |
dc.citation.number | 24 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | CARDIOMYOCYTES | - |
dc.subject.keywordPlus | CONVERSION | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | DISSOCIATION | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | KIDNEY EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | MESENCHYME | - |
dc.subject.keywordPlus | MOUSE FIBROBLASTS | - |
dc.subject.keywordPlus | PLURIPOTENT STEM-CELLS | - |
dc.subject.keywordPlus | PODOCYTES | - |
dc.subject.keywordAuthor | direct reprogramming | - |
dc.subject.keywordAuthor | kidney | - |
dc.subject.keywordAuthor | nephron progenitor cells | - |
dc.subject.keywordAuthor | transdifferentiation | - |
dc.subject.keywordAuthor | urine cells | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.