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Hydrogel-based hybridization chain reaction (HCR) for detection of urinary exosomal miRNAs as a diagnostic tool of prostate cancer

Authors
Kim, JunbeomShim, Ji SungHan, Bo HoonKim, Hye JinPark, JaesungCho, Il-JooKang, Sung GuKang, Ji YoonBong, Ki WanChoi, Nakwon
Issue Date
11월-2021
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Keywords
Urinary exosome; miRNA; Hydrogel microparticle; Diagnostics; Prostate cancer
Citation
BIOSENSORS & BIOELECTRONICS, v.192
Indexed
SCIE
SCOPUS
Journal Title
BIOSENSORS & BIOELECTRONICS
Volume
192
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/135728
DOI
10.1016/j.bios.2021.113504
ISSN
0956-5663
Abstract
Although urinary exosomal microRNAs (miRNAs) have recently emerged as potential biomarkers, clinical applications are still limited due to their low concentration in small volumes of clinical samples. Therefore, the development of a non-invasive, specific diagnostic tool, along with profiling exosomal miRNA markers from urine, remains a significant challenge. Here, we present hydrogel-based hybridization chain reaction (HCR) for multiplex signal amplification to detect urinary exosomal miRNAs from human clinical samples. We succeeded in identifying small amounts (similar to amol) of exosomal miRNAs from 600 mu L of urine with up to similar to 35-fold amplification and enhanced detection limits by over an order of magnitude for two miRNA biomarker candidates, hsa-miR6090 and hsa-miR-3665. Furthermore, we proposed ratiometric analysis without requiring normalization to a reference miRNA and validated the clinical diagnostic potential toward differentiating prostate cancer patients from healthy controls. Our hydrogel-based HCR could serve as a new diagnostic platform for a non-invasive liquid biopsy before burdensome tissue biopsy of various diseases, including prostate cancer screening, complementing the PSA test.
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