Long-term seasonal and temporal changes of hydrogen peroxide from cyanobacterial blooms in fresh waters

Abstract

In water, hydrogen peroxide (H2O2) is produced through abiotic and biotic reactions with organic matter, including algal cells. The production of H2O2 is influenced by harmful algal cell communities and toxicity. However, only a few studies have been conducted on H2O2 concentrations in natural water. Particularly, the seasonal and temporal patterns of H2O2 concentration suggest that H2O2 generation from aquatic microorganisms could be identified to compare of photochemical production from dissolved organic matter. Study area is a source of raw water and is a large artificial lake located near a metropolitan city. Due to various environmental conditions, harmful algal blooms frequently occur in summer. The purpose of this study was to trace the H2O2 concentration and water quality parameters of study area where algal bloom occurs and what factors directly affect the H2O2 concentration. Experiments were performed on the influencing factors via water samples from study area and lab-scale culture tank. The lake produces an average of 553 nM H2O2, which increases by more than three times (1460 nM) in summer compared the winter. The lake (18.6-23.8 nMh(-1)) produced more H2O2 than streams (7.4-9.0 nMh(-1)) during daylight hours. All water sites presented the lowest production rates in dark conditions (1.1-1.5 nMh(-1)). Daytime environment increased the generation rate more than the nighttime. The trend of H2O2 produced by algal cells was similar to that of the growth of algal cells. The exposure to external substances (heavy metals and antibiotics) increased the incidence by approximately five times; antibiotics were more influential than heavy metals.