A rapid quantitative on-site coronavirus disease 19 serological test
DC Field | Value | Language |
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dc.contributor.author | Lee, Jeong Hoon | - |
dc.contributor.author | Bae, Pan Kee | - |
dc.contributor.author | Kim, Hyunho | - |
dc.contributor.author | Song, Yoon Ji | - |
dc.contributor.author | Yi, So Yeon | - |
dc.contributor.author | Kwon, Jungsun | - |
dc.contributor.author | Seo, Joon-Seok | - |
dc.contributor.author | Lee, Jeong-min | - |
dc.contributor.author | Jo, Han-Sang | - |
dc.contributor.author | Park, Seon Mee | - |
dc.contributor.author | Park, Hee Sue | - |
dc.contributor.author | Shin, Kyeong Seob | - |
dc.contributor.author | Chung, Seok | - |
dc.contributor.author | Shin, Yong Beom | - |
dc.date.accessioned | 2022-02-14T23:40:33Z | - |
dc.date.available | 2022-02-14T23:40:33Z | - |
dc.date.created | 2022-02-08 | - |
dc.date.issued | 2021-11-01 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/135796 | - |
dc.description.abstract | On-site severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) serological assays allow for timely in-field decisions to be made regarding patient status, also enabling population-wide screening to assist in controlling the coronavirus disease 2019 (COVID-19) pandemic. Here we propose a rapid microfluidic serological assay with two unique functions of nanointerstice filling and digitized flow control, which enable the fast/robust filling of the sample fluid as well as precise regulation of duration and volume of immune reaction. Developed microfluidic assay showed enhanced limit of detection, and 91.67% sensitivity and 100% specificity (n = 152) for clinical samples of SARS CoV-2 patients. The assay enables daily monitoring of IgM/IgG titers and patterns, which could be crucial parameters for convalescence from COVID-19 and provide important insight into how the immune system responds to SARS CoV-2. The developed on-site microfluidic assay presented the mean time for IgM and IgG seroconversions, indicating that these titers plateaued days after seroconversion. The mean duration from day 0 to PCR negativity was 19.4 days (median 20 d, IQR 16-21 d), with higher IgM/IgG titres being observed when PCR positive turns into negative. Simple monitoring of these titres promotes rapid on-site detection and comprehensive understanding of the immune response of COVID-19 patients. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | A rapid quantitative on-site coronavirus disease 19 serological test | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Seok | - |
dc.identifier.doi | 10.1016/j.bios.2021.113406 | - |
dc.identifier.scopusid | 2-s2.0-85108371432 | - |
dc.identifier.wosid | 000685993400007 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.191 | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 191 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordAuthor | SARS-CoV-2 | - |
dc.subject.keywordAuthor | COVID-19 | - |
dc.subject.keywordAuthor | POCT | - |
dc.subject.keywordAuthor | Microfluidics | - |
dc.subject.keywordAuthor | Immunoassay | - |
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