Adenine base editor engineering reduces editing of bystander cytosines
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong, You Kyeong | - |
dc.contributor.author | Lee, SeokHoon | - |
dc.contributor.author | Hwang, Gue-Ho | - |
dc.contributor.author | Hong, Sung-Ah | - |
dc.contributor.author | Park, Se-eun | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.contributor.author | Woo, Jae-Sung | - |
dc.contributor.author | Bae, Sangsu | - |
dc.date.accessioned | 2022-02-15T06:41:42Z | - |
dc.date.available | 2022-02-15T06:41:42Z | - |
dc.date.created | 2022-02-09 | - |
dc.date.issued | 2021-11 | - |
dc.identifier.issn | 1087-0156 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/135830 | - |
dc.description.abstract | Adenine base editors (ABEs) catalyze specific A-to-G conversions at genomic sites of interest. However, ABEs also induce cytosine deamination at the target site. To reduce the cytosine editing activity, we engineered a commonly used adenosine deaminase, TadA7.10, and found that ABE7.10 with a D108Q mutation in TadA7.10 exhibited tenfold reduced cytosine deamination activity. The D108Q mutation also reduces cytosine deamination activity in two recently developed high-activity versions of ABE, ABE8e and ABE8s, and is compatible with V106W, a mutation that reduces off-target RNA editing. ABE7.10 containing a P48R mutation displayed increased cytosine deamination activity and a substantially reduced adenine editing rate, yielding a TC-specific base editing tool for TC-to-TT or TC-to-TG conversions that broadens the utility of base editors. Engineered variants of adenine base editors have reduced cytosine base editing or a specific C-to-G base editing activity. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE PORTFOLIO | - |
dc.subject | DNA | - |
dc.subject | MUTATIONS | - |
dc.subject | TARGET | - |
dc.title | Adenine base editor engineering reduces editing of bystander cytosines | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Woo, Jae-Sung | - |
dc.identifier.doi | 10.1038/s41587-021-00943-2 | - |
dc.identifier.scopusid | 2-s2.0-85109254847 | - |
dc.identifier.wosid | 000668816800001 | - |
dc.identifier.bibliographicCitation | NATURE BIOTECHNOLOGY, v.39, no.11, pp.1426 - + | - |
dc.relation.isPartOf | NATURE BIOTECHNOLOGY | - |
dc.citation.title | NATURE BIOTECHNOLOGY | - |
dc.citation.volume | 39 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1426 | - |
dc.citation.endPage | + | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.subject.keywordPlus | TARGET | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.