Near-infrared fluorescent imaging with indocyanine green in rabbit and patient specimens of esophageal cancer
- Authors
- Rho, Jiyun; Quan, Yu Hua; Choi, Byeong Hyeon; Han, Kook Nam; Kim, Beop-Min; Choi, Young Ho; Kim, Hyun Koo
- Issue Date
- 11월-2021
- Publisher
- AME PUBL CO
- Keywords
- Esophageal cancer; indocyanine green (ICG); translational research
- Citation
- JOURNAL OF THORACIC DISEASE, v.13, no.11, pp.6314 - +
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF THORACIC DISEASE
- Volume
- 13
- Number
- 11
- Start Page
- 6314
- End Page
- +
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/135905
- DOI
- 10.21037/jtd-21-790
- ISSN
- 2072-1439
- Abstract
- Background: We aimed to assess the possibility of detecting esophageal cancer after intravenous injection of indocyanine green (ICG) in preclinical and clinical models. Methods: Forty-five rabbits were surgically implanted with VX2 tumors into the esophageal muscular layer 2 weeks before esophagectomy. The rabbits received intravenous injection of ICG at doses of 1, 2, or 5 mg/kg at 3, 6, 12, 24, or 48 h before surgical removal of esophagus. Twelve patients scheduled to undergo esophagectomy were also enrolled, and all received 2 mg/kg of ICG intravenously at 3, 6, 12, or 24 h before surgical removal of esophagus. The fluorescence intensity was measured in all resected specimens from the rabbits and patients using a near-infrared (NIR) fluorescence imaging system after surgery. Results: Esophageal tumors were successfully established in all rabbits, and fluorescent signals were detected in all animal and patient specimens. Tumor-to-normal ratio (TNR) analysis showed that higher doses resulted in a greater TNR. Injection of at least 2 mg/kg of ICG was required for clear visualization of the tumor, and the TNR was highest at 12 h after injection. The TNR in patients was also highest at 12 h (P=0.0004), with 2 mg/kg of ICG. None of the patients had major complications following ICG injection. Conclusions: NIR fluorescence imaging can be used to visualize esophageal cancer after systemic injection of ICG. ICG at 2 mg/kg at 12 h is optimal for tumor detection. However, since the clinical trials were conducted in a small number of patients, further studies are needed in larger populations.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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