Augmented CO2 tolerance by expressing a single H+-pump enables microalgal valorization of industrial flue gas
DC Field | Value | Language |
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dc.contributor.author | Choi, Hong Il | - |
dc.contributor.author | Hwang, Sung-Won | - |
dc.contributor.author | Kim, Jongrae | - |
dc.contributor.author | Park, Byeonghyeok | - |
dc.contributor.author | Jin, EonSeon | - |
dc.contributor.author | Choi, In-Geol | - |
dc.contributor.author | Sim, Sang Jun | - |
dc.date.accessioned | 2022-02-16T22:41:24Z | - |
dc.date.available | 2022-02-16T22:41:24Z | - |
dc.date.created | 2022-02-08 | - |
dc.date.issued | 2021-10-18 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/136033 | - |
dc.description.abstract | Microalgae used for CO2 removal in an industrial exhaust gas stream usually has low CO2 tolerance. Here, the authors increase CO2 tolerance by expressing a single H + -pump and enable microalgal valorization of industrial flue gas. Microalgae can accumulate various carbon-neutral products, but their real-world applications are hindered by their CO2 susceptibility. Herein, the transcriptomic changes in a model microalga, Chlamydomonas reinhardtii, in a high-CO2 milieu (20%) are evaluated. The primary toxicity mechanism consists of aberrantly low expression of plasma membrane H+-ATPases (PMAs) accompanied by intracellular acidification. Our results demonstrate that the expression of a universally expressible PMA in wild-type strains makes them capable of not only thriving in acidity levels that they usually cannot survive but also exhibiting 3.2-fold increased photoautotrophic production against high CO2 via maintenance of a higher cytoplasmic pH. A proof-of-concept experiment involving cultivation with toxic flue gas (13 vol% CO2, 20 ppm NOX, and 32 ppm SOX) shows that the production of CO2-based bioproducts by the strain is doubled compared with that by the wild-type, implying that this strategy potentially enables the microalgal valorization of CO2 in industrial exhaust. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE PORTFOLIO | - |
dc.subject | PLASMA-MEMBRANE | - |
dc.subject | CHLAMYDOMONAS-REINHARDTII | - |
dc.subject | HAEMATOCOCCUS-PLUVIALIS | - |
dc.subject | NICOTIANA-TABACUM | - |
dc.subject | ATP SYNTHASE | - |
dc.subject | POWER-PLANTS | - |
dc.subject | ALGA | - |
dc.subject | PROTEINS | - |
dc.subject | GENE | - |
dc.subject | CHLORELLA | - |
dc.title | Augmented CO2 tolerance by expressing a single H+-pump enables microalgal valorization of industrial flue gas | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, In-Geol | - |
dc.contributor.affiliatedAuthor | Sim, Sang Jun | - |
dc.identifier.doi | 10.1038/s41467-021-26325-5 | - |
dc.identifier.scopusid | 2-s2.0-85117620661 | - |
dc.identifier.wosid | 000708601800027 | - |
dc.identifier.bibliographicCitation | NATURE COMMUNICATIONS, v.12, no.1 | - |
dc.relation.isPartOf | NATURE COMMUNICATIONS | - |
dc.citation.title | NATURE COMMUNICATIONS | - |
dc.citation.volume | 12 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | ALGA | - |
dc.subject.keywordPlus | ATP SYNTHASE | - |
dc.subject.keywordPlus | CHLAMYDOMONAS-REINHARDTII | - |
dc.subject.keywordPlus | CHLORELLA | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | HAEMATOCOCCUS-PLUVIALIS | - |
dc.subject.keywordPlus | NICOTIANA-TABACUM | - |
dc.subject.keywordPlus | PLASMA-MEMBRANE | - |
dc.subject.keywordPlus | POWER-PLANTS | - |
dc.subject.keywordPlus | PROTEINS | - |
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