Immunostimulatory Potential of Extracellular Vesicles Isolated from an Edible Plant, Petasites japonicus, via the Induction of Murine Dendritic Cell Maturation
DC Field | Value | Language |
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dc.contributor.author | Han, Jeong Moo | - |
dc.contributor.author | Song, Ha-Yeon | - |
dc.contributor.author | Lim, Seung-Taik | - |
dc.contributor.author | Kim, Kwang-Il | - |
dc.contributor.author | Seo, Ho Seong | - |
dc.contributor.author | Byun, Eui-Baek | - |
dc.date.accessioned | 2022-02-17T17:40:33Z | - |
dc.date.available | 2022-02-17T17:40:33Z | - |
dc.date.created | 2022-02-09 | - |
dc.date.issued | 2021-10 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/136126 | - |
dc.description.abstract | Extracellular vesicles (EVs) have recently been isolated from different plants. Plant-derived EVs have been proposed as potent therapeutics and drug-delivery nanoplatforms for delivering biomolecules, including proteins, RNAs, DNAs, and lipids. Herein, Petasites japonicus-derived EVs (PJ-EVs) were isolated through a series of centrifugation steps and characterized using dynamic light scattering and transmission electron microscopy. Immunomodulatory effects of PJ-EVs were assessed using dendritic cells (DCs). PJ-EVs exhibited a spherical morphology with an average size of 122.6 nm. They induced the maturation of DCs via an increase in the expression of surface molecules (CD80, CD86, MHC-I, and MHC-II), production of Th1-polarizing cytokines (TNF-a and IL-12p70), and antigen-presenting ability; however, they reduced the antigen-uptake ability. Furthermore, maturation of DCs induced by PJ-EVs was dependent on the activation and phosphorylation of MAPK and NF-kappa B signal pathways. Notably, PJ-EV-treated DCs strongly induced the proliferation and differentiation of naive T cells toward Th1-type T cells and cytotoxic CD8(+) T cells along with robust secretion of IFN-gamma and IL-2. In conclusion, our study indicates that PJ-EVs can be potent immunostimulatory candidates with an ability of strongly inducing the maturation of DCs. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | CANCER | - |
dc.subject | NANOPARTICLES | - |
dc.subject | RESPONSES | - |
dc.subject | EXTRACTS | - |
dc.title | Immunostimulatory Potential of Extracellular Vesicles Isolated from an Edible Plant, Petasites japonicus, via the Induction of Murine Dendritic Cell Maturation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lim, Seung-Taik | - |
dc.identifier.doi | 10.3390/ijms221910634 | - |
dc.identifier.scopusid | 2-s2.0-85116056809 | - |
dc.identifier.wosid | 000710241300001 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.22, no.19 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 22 | - |
dc.citation.number | 19 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | EXTRACTS | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | RESPONSES | - |
dc.subject.keywordAuthor | Petasites japonicus | - |
dc.subject.keywordAuthor | T cells | - |
dc.subject.keywordAuthor | dendritic cells | - |
dc.subject.keywordAuthor | extracellular vesicles | - |
dc.subject.keywordAuthor | immunostimulatory | - |
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