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Generation of an ACTA2-EGFP reporter human induced pluripotent stem cell line, KITi001-C-41, using CRISPR/Cas9-mediated homologous recombination

Authors
Jo, SeongyeaKim, Ji-WooNoh, HaneulKim, HyeminKim, Jong-HoonPark, Han-Jin
Issue Date
10월-2021
Publisher
ELSEVIER
Citation
STEM CELL RESEARCH, v.56
Indexed
SCIE
SCOPUS
Journal Title
STEM CELL RESEARCH
Volume
56
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/136147
DOI
10.1016/j.scr.2021.102524
ISSN
1873-5061
Abstract
Alpha-smooth muscle actin (alpha-SMA) is encoded by ACTA2 and is a key protein in the cellular contractile system of various mesodermal cell types, including hepatic stellate cells (HSCs), smooth muscle cells, and cardiomyocytes. alpha-SMA, which is a key protein in the development of hepatic fibrosis, is widely used as a reliable marker of HSC activation. Here, we generated an ACTA2-EGFP reporter human induced pluripotent stem cell line, KITi001-C-41, using a CRISPR/Cas9-based knock-in system. These reporter hiPSC lines can be used for lineage tracing of mesodermal cells and for screening of HSC activation factors.
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