Compensatory Protection of Thioredoxin-Deficient Cells from Etoposide-Induced Cell Death by Selenoprotein W via Interaction with 14-3-3
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Hyunwoo | - |
dc.contributor.author | Jeon, Yeong Ha | - |
dc.contributor.author | Ham, Minju | - |
dc.contributor.author | Ko, Kwanyoung | - |
dc.contributor.author | Kim, Ick Young | - |
dc.date.accessioned | 2022-02-17T23:41:00Z | - |
dc.date.available | 2022-02-17T23:41:00Z | - |
dc.date.created | 2022-02-08 | - |
dc.date.issued | 2021-10 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/136158 | - |
dc.description.abstract | Selenoprotein W (SELENOW) is a 9.6 kDa protein containing selenocysteine (Sec, U) in a conserved Cys-X-X-Sec (CXXU) motif. Previously, we reported that SELENOW regulates various cellular processes by interacting with 14-3-3 beta at the U of the CXXU motif. Thioredoxin (Trx) is a small protein that plays a key role in the cellular redox regulatory system. The CXXC motif of Trx is critical for redox regulation. Recently, an interaction between Trx1 and 14-3-3 has been predicted. However, the binding mechanism and its biological effects remain unknown. In this study, we found that Trx1 interacted with 14-3-3 beta at the Cys32 residue in the CXXC motif, and SELENOW and Trx1 were bound at Cys191 residue of 14-3-3 beta. In vitro binding assays showed that SELENOW and Trx1 competed for interaction with 14-3-3 beta. Compared to control cells, Trx1-deficient cells and SELENOW-deficient cells showed increased levels of both the subG1 population and poly (ADP-ribose) polymerase (PARP) cleavage by etoposide treatment. Moreover, Akt phosphorylation of Ser473 was reduced in Trx1-deficient cells and was recovered by overexpression of SELENOW. These results indicate that SELENOW can protect Trx1-deficient cells from etoposide-induced cell death through its interaction with 14-3-3 beta. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | ACTS DOWNSTREAM | - |
dc.subject | PROTEINS | - |
dc.subject | BINDING | - |
dc.subject | SYSTEM | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | IDENTIFICATION | - |
dc.subject | GLUTATHIONE | - |
dc.subject | ACTIVATION | - |
dc.subject | REDUCTASE | - |
dc.subject | TARGET | - |
dc.title | Compensatory Protection of Thioredoxin-Deficient Cells from Etoposide-Induced Cell Death by Selenoprotein W via Interaction with 14-3-3 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Ick Young | - |
dc.identifier.doi | 10.3390/ijms221910338 | - |
dc.identifier.scopusid | 2-s2.0-85115720697 | - |
dc.identifier.wosid | 000709472400001 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.22, no.19 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 22 | - |
dc.citation.number | 19 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | ACTS DOWNSTREAM | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | GLUTATHIONE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | REDUCTASE | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | TARGET | - |
dc.subject.keywordAuthor | 14-3-3 | - |
dc.subject.keywordAuthor | Akt | - |
dc.subject.keywordAuthor | cell death | - |
dc.subject.keywordAuthor | selenoprotein W | - |
dc.subject.keywordAuthor | thioredoxin | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.