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Multimodal microscopy for the simultaneous visualization of five different imaging modalities using a single light source

Authors
Ryu, JiheunKang, UnkyoSong, Joon WooKim, JunyoungKim, Jin WonYoo, HongkiGweon, Bomi
Issue Date
1-9월-2021
Publisher
OPTICAL SOC AMER
Citation
BIOMEDICAL OPTICS EXPRESS, v.12, no.9, pp.5452 - 5469
Indexed
SCIE
SCOPUS
Journal Title
BIOMEDICAL OPTICS EXPRESS
Volume
12
Number
9
Start Page
5452
End Page
5469
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/136407
DOI
10.1364/BOE.430677
ISSN
2156-7085
Abstract
Optical microscopy has been widely used in biomedical research as it provides photophysical and photochemical information of the target in subcellular spatial resolution without requiring physical contact with the specimen. To obtain a deeper understanding of biological phenomena, several efforts have been expended to combine such optical imaging modalities into a single microscope system. However, the use of multiple light sources and detectors through separated beam paths renders previous systems extremely complicated or slow for in vivo imaging. Herein, we propose a novel high-speed multimodal optical microscope system that simultaneously visualizes five different microscopic contrasts, i.e., two-photon excitation, second-harmonic generation, backscattered light, near-infrared fluorescence, and fluorescence lifetime, using a single femtosecond pulsed laser. Our proposed system can visualize five modal images with a frame rate of 3.7 fps in real-time, thereby providing complementary optical information that enhances both structural and functional contrasts. This highly photon-efficient multimodal microscope system enables various properties of biological tissues to be assessed. (c) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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