Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Hevin-calcyon interaction promotes synaptic reorganization after brain injury

Authors
Kim, Jong-HeonJung, Hyun-GugKim, AjungShim, Hyun SooHyeon, Seung JaeLee, Young-SunHan, JinJung, Jong HoonLee, JaekwangRyu, HoonPark, Jae-YongHwang, Eun MiSuk, Kyoungho
Issue Date
9월-2021
Publisher
SPRINGERNATURE
Citation
CELL DEATH AND DIFFERENTIATION, v.28, no.9, pp.2571 - 2588
Indexed
SCIE
SCOPUS
Journal Title
CELL DEATH AND DIFFERENTIATION
Volume
28
Number
9
Start Page
2571
End Page
2588
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/136410
DOI
10.1038/s41418-021-00772-5
ISSN
1350-9047
Abstract
Hevin, also known as SPARC-like protein 1 (SPARCL1 or SC1), is a synaptogenic protein secreted by astrocytes and modulates the formation of glutamatergic synapses in the developing brain by interacting with synaptic adhesion proteins, such as neurexin and neuroligin. Here, we identified the neuron-specific vesicular protein calcyon as a novel interaction partner of hevin and demonstrated that this interaction played a pivotal role in synaptic reorganization after an injury in the mature brain. Astrocytic hevin was upregulated post-injury in a photothrombotic stroke model. Hevin was fragmented by MMP3 induced during the acute stage of brain injury, and this process was associated with severe gliosis. At the late stage, the functional hevin level was restored as MMP3 expression decreased. The C-terminus of hevin interacted with the N-terminus of calcyon. By using RNAi and binding competitor peptides in an ischemic brain injury model, we showed that this interaction was crucial in synaptic and functional recoveries in the sensory-motor cortex, based on histological and electrophysiological analyses. Regulated expression of hevin and calcyon and interaction between them were confirmed in a mouse model of traumatic brain injury and patients with chronic traumatic encephalopathy. Our study provides direct evidence for the causal relationship between the hevin-calcyon interaction and synaptic reorganization after brain injury. This neuron-glia interaction can be exploited to modulate synaptic reorganization under various neurological conditions.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE