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Thymidine decreases the DNA damage and apoptosis caused by tumor-treating fields in cancer cell lines

Authors
Jeong, HyesunJo, YunhuiYoon, MyonggeunHong, Sunghoi
Issue Date
9월-2021
Publisher
SPRINGER
Keywords
Tumor& #8208; treating fields; cancer cell; DNA damage; Cell division; Thymidine
Citation
GENES & GENOMICS, v.43, no.9, pp.995 - 1001
Indexed
SCIE
SCOPUS
KCI
Journal Title
GENES & GENOMICS
Volume
43
Number
9
Start Page
995
End Page
1001
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/136434
DOI
10.1007/s13258-021-01105-z
ISSN
1976-9571
Abstract
Background Tumor-treating fields (TTFields) is an emerging non-invasive cancer-treatment modality using alternating electric fields with low intensities and an intermediate range of frequency. TTFields affects an extensive range of charged and polarizable cellular factors known to be involved in cell division. However, it causes side-effects, such as DNA damage and apoptosis, in healthy cells. Objective To investigate whether thymidine can have an effect on the DNA damage and apoptosis, we arrested the cell cycle of human glioblastoma cells (U373) at G1/S phase by using thymidine and then exposed these cells to TTFields. Methods Cancer cell lines and normal cell (HaCaT) were arrested by thymidine double block method. Cells were seeded into the gap of between the insulated wires. The exposed in alternative electric fields at 120 kHz, 1.2 V/cm. They were counted the cell numbers and analyzed for cancer malignant such as colony formation, Annexin V/PI staining, gamma H2AX and RT-PCR. Results The colony-forming ability and DNA damage of the control cells without thymidine treatment were significantly decreased, and the expression levels of BRCA1, PCNA, CDC25C, and MAD2 were distinctly increased. Interestingly, however, cells treated with thymidine did not change the colony formation, apoptosis, DNA damage, or gene expression pattern. Conclusions These results demonstrated that thymidine can inhibit the TTFields-caused DNA damage and apoptosis, suggesting that combining TTFields and conventional treatments, such as chemotherapy, may enhance prognosis and decrease side effects compared with those of TTFields or conventional treatments alone.
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Yoon, Myong geun
보건과학대학 (바이오의공학부)
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