Effects of mechanical properties of gelatin methacryloyl hydrogels on encapsulated stem cell spheroids for 3D tissue engineering
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Eun Mi | - |
dc.contributor.author | Lee, Gyeong Min | - |
dc.contributor.author | Lee, Sangmin | - |
dc.contributor.author | Kim, Se-jeong | - |
dc.contributor.author | Lee, Dongtak | - |
dc.contributor.author | Yoon, Dae Sung | - |
dc.contributor.author | Joo, Jinmyoung | - |
dc.contributor.author | Kong, Hyunjoon | - |
dc.contributor.author | Park, Hee Ho | - |
dc.contributor.author | Shin, Heungsoo | - |
dc.date.accessioned | 2022-02-22T20:41:53Z | - |
dc.date.available | 2022-02-22T20:41:53Z | - |
dc.date.created | 2022-02-11 | - |
dc.date.issued | 2022-01-01 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/136540 | - |
dc.description.abstract | Cell spheroids are three-dimensional cell aggregates that have been widely employed in tissue engineering. Spheroid encapsulation has been explored as a method to enhance cell-cell interactions. However, the effect hydrogel mechanical properties on spheroids, specifically soft hydrogels (<1 kPa), has not yet been studied. this study, we determined the effect of encapsulation of stem cell spheroids by hydrogels crosslinked with different concentrations of gelatin methacryloyl (GelMA) on the functions of the stem cells. To this end, human adipose-derived stem cell (ADSC) spheroids with a defined size were prepared, and spheroid-laden hydrogels with various concentrations (5, 10, 15%) were fabricated. The apoptotic index of cells from spheroids encapsulated in the 15% hydrogel was high. The migration distance was five-fold higher in cells encapsulated in the 5% hydrogel than the 10% hydrogel. After 14 days of culture, cells from spheroids in the 5% hydrogel were observed to have spread and proliferated. Osteogenic factor and pro-angiogenic factor production in the 15% hydrogel was high. Collectively, our results indicate that the functionality of spheroids can be regulated by the mechanical properties of hydrogel, even under 1 kPa. These results indicate that spheroid-laden hydrogels are suitable for use in 3D tissue construction. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.subject | OSTEOGENIC DIFFERENTIATION | - |
dc.subject | E-CADHERIN | - |
dc.subject | PROLIFERATION | - |
dc.subject | MIGRATION | - |
dc.subject | CULTURE | - |
dc.subject | CONNEXIN-43 | - |
dc.subject | INTERPLAY | - |
dc.subject | MODULUS | - |
dc.subject | SURFACE | - |
dc.title | Effects of mechanical properties of gelatin methacryloyl hydrogels on encapsulated stem cell spheroids for 3D tissue engineering | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yoon, Dae Sung | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2021.11.145 | - |
dc.identifier.scopusid | 2-s2.0-85120404656 | - |
dc.identifier.wosid | 000734362300002 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.194, pp.903 - 913 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.citation.volume | 194 | - |
dc.citation.startPage | 903 | - |
dc.citation.endPage | 913 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | OSTEOGENIC DIFFERENTIATION | - |
dc.subject.keywordPlus | E-CADHERIN | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | CONNEXIN-43 | - |
dc.subject.keywordPlus | INTERPLAY | - |
dc.subject.keywordPlus | MODULUS | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordAuthor | Stem cell spheroid | - |
dc.subject.keywordAuthor | Gelatin methacryloyl | - |
dc.subject.keywordAuthor | Encapsulation | - |
dc.subject.keywordAuthor | Spheroid-laden hydrogel | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.