Genotyping and Molecular Diagnosis of Hepatitis A Virus in Human Clinical Samples Using Multiplex PCR-Based Next-Generation Sequencing
DC Field | Value | Language |
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dc.contributor.author | Lee, Geum-Young | - |
dc.contributor.author | Kim, Won-Keun | - |
dc.contributor.author | Cho, Seungchan | - |
dc.contributor.author | Park, Kyungmin | - |
dc.contributor.author | Kim, Jongwoo | - |
dc.contributor.author | Lee, Seung-Ho | - |
dc.contributor.author | Lee, Jingyeong | - |
dc.contributor.author | Lee, Young-Sun | - |
dc.contributor.author | Kim, Ji Hoon | - |
dc.contributor.author | Byun, Kwan Soo | - |
dc.contributor.author | Song, Jin-Won | - |
dc.date.accessioned | 2022-02-22T22:42:09Z | - |
dc.date.available | 2022-02-22T22:42:09Z | - |
dc.date.created | 2022-02-15 | - |
dc.date.issued | 2022-01 | - |
dc.identifier.issn | 2076-2607 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/136552 | - |
dc.description.abstract | Hepatitis A virus (HAV) is a serious threat to public health worldwide. We used multiplex polymerase chain reaction (PCR)-based next-generation sequencing (NGS) to derive information on viral genetic diversity and conduct precise phylogenetic analysis. Four HAV genome sequences were obtained using multiplex PCR-based NGS. HAV whole-genome sequence of one sample was obtained by conventional Sanger sequencing. The HAV strains demonstrated a geographic cluster with sub-genotype IA strains in the Republic of Korea. The phylogenetic pattern of HAV viral protein (VP) 3 region showed no phylogenetic conflict between the whole-genome and partial-genome sequences. The VP3 region in serum and stool samples showed sensitive detection of HAV with differences of quantification that did not exceed <10 copies/mu L than the consensus VP4 region using quantitative PCR (qPCR). In conclusion, multiplex PCR-based NGS was implemented to define HAV genotypes using nearly whole-genome sequences obtained directly from hepatitis A patients. The VP3 region might be a potential candidate for tracking the genotypic origin of emerging HAV outbreaks. VP3-specific qPCR was developed for the molecular diagnosis of HAV infection. This study may be useful to predict for the disease management and subsequent development of hepatitis A infection at high risk of severe illness. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | FULL-LENGTH GENOME | - |
dc.subject | GENETIC RELATEDNESS | - |
dc.subject | UNITED-STATES | - |
dc.subject | OUTBREAK | - |
dc.subject | EPIDEMIOLOGY | - |
dc.subject | SEX | - |
dc.subject | MEN | - |
dc.subject | EVOLUTION | - |
dc.subject | POPULATIONS | - |
dc.subject | INFECTIONS | - |
dc.title | Genotyping and Molecular Diagnosis of Hepatitis A Virus in Human Clinical Samples Using Multiplex PCR-Based Next-Generation Sequencing | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Jin-Won | - |
dc.identifier.doi | 10.3390/microorganisms10010100 | - |
dc.identifier.scopusid | 2-s2.0-85122055992 | - |
dc.identifier.wosid | 000749503700001 | - |
dc.identifier.bibliographicCitation | MICROORGANISMS, v.10, no.1 | - |
dc.relation.isPartOf | MICROORGANISMS | - |
dc.citation.title | MICROORGANISMS | - |
dc.citation.volume | 10 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | FULL-LENGTH GENOME | - |
dc.subject.keywordPlus | GENETIC RELATEDNESS | - |
dc.subject.keywordPlus | UNITED-STATES | - |
dc.subject.keywordPlus | OUTBREAK | - |
dc.subject.keywordPlus | EPIDEMIOLOGY | - |
dc.subject.keywordPlus | SEX | - |
dc.subject.keywordPlus | MEN | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | POPULATIONS | - |
dc.subject.keywordPlus | INFECTIONS | - |
dc.subject.keywordAuthor | hepatitis A virus | - |
dc.subject.keywordAuthor | multiplex polymerase chain reaction | - |
dc.subject.keywordAuthor | next-generation sequencing | - |
dc.subject.keywordAuthor | phylogenetic analysis | - |
dc.subject.keywordAuthor | genotypic analysis | - |
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