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Aptamer duo-based portable electrochemical biosensors for early diagnosis of periodontal disease

Authors
Joe, C.Lee, B.H.Kim, S.H.Ko, Y.Gu, M.B.
Issue Date
1-3월-2022
Publisher
Elsevier Ltd
Keywords
Aptamer duo; Human odontogenic ameloblast-associated protein (ODAM); Portable electrochemical aptasensor; Sandwich-type binding
Citation
Biosensors and Bioelectronics, v.199
Indexed
SCIE
SCOPUS
Journal Title
Biosensors and Bioelectronics
Volume
199
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/136642
DOI
10.1016/j.bios.2021.113884
ISSN
0956-5663
Abstract
Recently, point-of-care tests (POCT) have gained much attention due to their convenient, fast, simple, and easy characteristics. For POCT, portability is an essential feature. In this study, we have successfully fabricated a portable mini-potentiostat. Using chronoamperometry, electrical signals of this portable mini-potentiostat were measured, and the analytical performance of electrochemical aptasensors was compared with a benchtop potentiostat. The electrochemical signals measured by mini-potentiostat can be displayed on the screen of a smartphone. To verify the analytical performance of this portable electrochemical aptasensor platform with a mini-potentiostat, two well-known model protein biomarkers, vaspin, a type 2 diabetes biomarker, and thrombin, a biomarker for pulmonary metastasis and cardiovascular disease, were confirmed to be detected by using corresponding aptamer duo. After solid verification of this portable electrochemical aptasensor platform, we have successfully implemented this portable mini-potentiostat system to develop a portable sandwich-type binding pair of aptamers-based electrochemical biosensor, which can diagnose periodontal disease by measuring ODAM biomarker. The linear range of this ODAM biosensor was 0 to 15 nM with a detection limit of 0.02 nM and 1 nM in buffer and saliva, respectively. The sensitivity of this biosensor has been greatly enhanced, compared to previously developed surface plasmon resonance (SPR) or lateral flow assay (LFA) based aptasensors. This study showed that this new portable aptamer duo-based biosensor is expected to diagnose the early stage of periodontal diseases from real samples, such as saliva or gingival crevicular fluid in a short time as a point-of-care (POC) testing. © 2021 Elsevier B.V.
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