uORF-mediated riboregulation controls transcription of whiB7/wblC antibiotic resistance gene
DC Field | Value | Language |
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dc.contributor.author | Lee, Ju-Hyung | - |
dc.contributor.author | Lee, Eun-Jin | - |
dc.contributor.author | Roe, Jung-Hye | - |
dc.date.accessioned | 2022-02-23T19:40:24Z | - |
dc.date.available | 2022-02-23T19:40:24Z | - |
dc.date.created | 2022-02-11 | - |
dc.date.issued | 2022-01 | - |
dc.identifier.issn | 0950-382X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/136654 | - |
dc.description.abstract | WhiB7/WblC is a transcriptional factor of actinomycetes conferring intrinsic resistance to multiple translation-inhibitory antibiotics. It positively autoregulates its own transcription in response to the same antibiotics. The presence of a uORF and a potential Rho-independent transcription terminator in the 5 ' leader region has suggested a possibility that the whiB7/wblC gene is regulated via a uORF-mediated transcription attenuation. However, experimental evidence for the molecular mechanism to explain how antibiotic stress suppresses the attenuator, if any, and induces transcription of the whiB7/wblC gene has been lacking. Here we report that the 5 ' leader sequences of the whiB7/wblC genes in sub-clades of actinomycetes include conserved antiterminator RNA structures. We confirmed that the putative antiterminator in the whiB7/wblC leader sequences of both Streptomyces and Mycobacterium indeed suppresses Rho-independent transcription terminator and facilitates transcription readthrough, which is required for WhiB7/WblC-mediated antibiotic resistance. The antibiotic-mediated suppression of the attenuator can be recapitulated by amino acid starvation, indicating that translational inhibition of uORF by multiple signals is a key to induce whiB7/wblC expression. Our findings of a mechanism leading to intrinsic antibiotic resistance could provide an alternative to treat drug-resistant mycobacteria. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | MYCOBACTERIUM-TUBERCULOSIS | - |
dc.subject | DRUG-RESISTANCE | - |
dc.subject | EF-P | - |
dc.subject | RIBOSOME | - |
dc.subject | STREPTOMYCES | - |
dc.subject | ATTENUATION | - |
dc.subject | MUTATIONS | - |
dc.subject | EXPRESSION | - |
dc.subject | REGULATOR | - |
dc.subject | KANAMYCIN | - |
dc.title | uORF-mediated riboregulation controls transcription of whiB7/wblC antibiotic resistance gene | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Eun-Jin | - |
dc.identifier.doi | 10.1111/mmi.14834 | - |
dc.identifier.scopusid | 2-s2.0-85118344228 | - |
dc.identifier.wosid | 000713761500001 | - |
dc.identifier.bibliographicCitation | MOLECULAR MICROBIOLOGY, v.117, no.1, pp.179 - 192 | - |
dc.relation.isPartOf | MOLECULAR MICROBIOLOGY | - |
dc.citation.title | MOLECULAR MICROBIOLOGY | - |
dc.citation.volume | 117 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 179 | - |
dc.citation.endPage | 192 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | MYCOBACTERIUM-TUBERCULOSIS | - |
dc.subject.keywordPlus | DRUG-RESISTANCE | - |
dc.subject.keywordPlus | EF-P | - |
dc.subject.keywordPlus | RIBOSOME | - |
dc.subject.keywordPlus | STREPTOMYCES | - |
dc.subject.keywordPlus | ATTENUATION | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | REGULATOR | - |
dc.subject.keywordPlus | KANAMYCIN | - |
dc.subject.keywordAuthor | antibiotic resistance | - |
dc.subject.keywordAuthor | antiterminator | - |
dc.subject.keywordAuthor | Mycobacterium | - |
dc.subject.keywordAuthor | Streptomyces | - |
dc.subject.keywordAuthor | transcription attenuation | - |
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