DMRT1 gene disruption alone induces incomplete gonad feminization in chicken
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Hong Jo | - |
dc.contributor.author | Seo, Minseok | - |
dc.contributor.author | Choi, Hee Jung | - |
dc.contributor.author | Rengaraj, Deivendran | - |
dc.contributor.author | Jung, Kyung Min | - |
dc.contributor.author | Park, Jin Se | - |
dc.contributor.author | Lee, Kyung Youn | - |
dc.contributor.author | Kim, Young Min | - |
dc.contributor.author | Park, Kyung Je | - |
dc.contributor.author | Han, Soo Taek | - |
dc.contributor.author | Lee, Kyu Hyuk | - |
dc.contributor.author | Yao, Humphrey Hung-Chang | - |
dc.contributor.author | Han, Jae Yong | - |
dc.date.accessioned | 2022-02-24T07:41:22Z | - |
dc.date.available | 2022-02-24T07:41:22Z | - |
dc.date.created | 2022-02-07 | - |
dc.date.issued | 2021-09 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/136718 | - |
dc.description.abstract | Compared with the well-described XY sex determination system in mammals, the avian ZW sex determination system is poorly understood. Knockdown and overexpression studies identified doublesex and mab-3-related transcription factor 1 (DMRT1) as the testis-determining gene in chicken. However, the detailed effects of DMRT1 gene disruption from embryonic to adult development are not clear. Herein, we have generated DMRT1-disrupted chickens using the clustered regularly interspaced short palindromic repeats-associated protein 9 system, followed by an analysis of physiological, hormonal, and molecular changes in the genome-modified chickens. In the early stages of male chicken development, disruption of DMRT1 induced gonad feminization with extensive physiological and molecular changes; however, functional feminine reproductivity could not be implemented with disturbed hormone synthesis. Subsequent RNA-sequencing analysis of the DMRT1-disrupted chicken gonads revealed gene networks, including several novel genes linearly and non-linearly associated with DMRT1, which are involved in gonad feminization. By comparing the gonads of wild type with the genome-modified chickens, a set of genes were identified that is involved in the ZW sex determination system independent of DMRT1. Our results extend beyond the Z-dosage hypothesis to provide further information about the avian ZW sex determination system and epigenetic effects of gonad feminization. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | EXPRESSION PATTERN | - |
dc.subject | SEX DETERMINATION | - |
dc.subject | NONCODING RNA | - |
dc.subject | MALE PATHWAY | - |
dc.subject | DNA-BINDING | - |
dc.subject | GERM-CELLS | - |
dc.subject | CHROMOSOME | - |
dc.subject | PROTEIN | - |
dc.subject | SUGGESTS | - |
dc.subject | REGION | - |
dc.title | DMRT1 gene disruption alone induces incomplete gonad feminization in chicken | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Seo, Minseok | - |
dc.identifier.doi | 10.1096/fj.202100902R | - |
dc.identifier.scopusid | 2-s2.0-85113442450 | - |
dc.identifier.wosid | 000691122900060 | - |
dc.identifier.bibliographicCitation | FASEB JOURNAL, v.35, no.9 | - |
dc.relation.isPartOf | FASEB JOURNAL | - |
dc.citation.title | FASEB JOURNAL | - |
dc.citation.volume | 35 | - |
dc.citation.number | 9 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | CHROMOSOME | - |
dc.subject.keywordPlus | DNA-BINDING | - |
dc.subject.keywordPlus | EXPRESSION PATTERN | - |
dc.subject.keywordPlus | GERM-CELLS | - |
dc.subject.keywordPlus | MALE PATHWAY | - |
dc.subject.keywordPlus | NONCODING RNA | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | REGION | - |
dc.subject.keywordPlus | SEX DETERMINATION | - |
dc.subject.keywordPlus | SUGGESTS | - |
dc.subject.keywordAuthor | DMRT1 | - |
dc.subject.keywordAuthor | chicken | - |
dc.subject.keywordAuthor | genome editing | - |
dc.subject.keywordAuthor | sex determination | - |
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