Translation mediated by the nuclear cap-binding complex is confined to the perinuclear region via a CTIF-DDX19B interaction
DC Field | Value | Language |
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dc.contributor.author | Park, Yeonkyoung | - |
dc.contributor.author | Park, Joori | - |
dc.contributor.author | Hwang, Hyun Jung | - |
dc.contributor.author | Kim, Leehyeon | - |
dc.contributor.author | Jeong, Kwon | - |
dc.contributor.author | Song, Hyun Kyu | - |
dc.contributor.author | Rufener, Simone C. | - |
dc.contributor.author | Muehlemann, Oliver | - |
dc.contributor.author | Kim, Yoon Ki | - |
dc.date.accessioned | 2022-02-25T06:40:44Z | - |
dc.date.available | 2022-02-25T06:40:44Z | - |
dc.date.created | 2022-02-07 | - |
dc.date.issued | 2021-08-20 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/136830 | - |
dc.description.abstract | Newly synthesized mRNA is translated during its export through the nuclear pore complex, when its 5'-cap structure is still bound by the nuclear cap-binding complex (CBC), a heterodimer of cap-binding protein (CBP) 80 and CBP20. Despite its critical role in mRNA surveillance, the mechanism by which CBC-dependent translation (CT) is regulated remains unknown. Here, we demonstrate that the CT initiation factor (CTIF) is tethered in a translationally incompetent manner to the perinuclear region by the DEAD-box helicase 19B (DDX19B). DDX19B hands over CTIF to CBP80, which is associated with the 5'-cap of a newly exported mRNA. The resulting CBP80-CTIF complex then initiates CT in the perinuclear region. We also show that impeding the interaction between CTIF and DDX19B leads to uncontrolled CT throughout the cytosol, consequently dysregulating nonsense-mediated mRNA decay. Altogether, our data provide molecular evidence supporting the importance of tight control of local translation in the perinuclear region. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.subject | MESSENGER-RNA DECAY | - |
dc.subject | NUCLEOPORIN NUP214 | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | HELICASE DDX19 | - |
dc.subject | PROTEIN | - |
dc.subject | EXPORT | - |
dc.subject | DBP5 | - |
dc.subject | INITIATION | - |
dc.subject | OCCURS | - |
dc.subject | IDENTIFICATION | - |
dc.title | Translation mediated by the nuclear cap-binding complex is confined to the perinuclear region via a CTIF-DDX19B interaction | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Hyun Kyu | - |
dc.contributor.affiliatedAuthor | Kim, Yoon Ki | - |
dc.identifier.doi | 10.1093/nar/gkab579 | - |
dc.identifier.scopusid | 2-s2.0-85113147075 | - |
dc.identifier.wosid | 000692599800038 | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, v.49, no.14, pp.8261 - 8276 | - |
dc.relation.isPartOf | NUCLEIC ACIDS RESEARCH | - |
dc.citation.title | NUCLEIC ACIDS RESEARCH | - |
dc.citation.volume | 49 | - |
dc.citation.number | 14 | - |
dc.citation.startPage | 8261 | - |
dc.citation.endPage | 8276 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | DBP5 | - |
dc.subject.keywordPlus | EXPORT | - |
dc.subject.keywordPlus | HELICASE DDX19 | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | INITIATION | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | MESSENGER-RNA DECAY | - |
dc.subject.keywordPlus | NUCLEOPORIN NUP214 | - |
dc.subject.keywordPlus | OCCURS | - |
dc.subject.keywordPlus | PROTEIN | - |
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