Aminipila terrae sp. nov., a strictly anaerobic bacterium isolated from river sediment

Abstract

In this study, aimed at investigating and characterizing river sediment bacteria, we isolated a Gram-stain-positive, rod-shaped, obligate anaerobic bacterium, strain CBA3637(T), from the sediment of the Geum River. This strain grew at 10-40 degrees C (optimum, 30 degrees C), 0-1% NaCl (optimum, 0%), and pH 7-8 (optimum, pH 7). The 16S rRNA gene sequence comparison revealed Aminipila butyrica DSM 103574(T) to be the closest relative of strain CBA3637(T) (96.6-96.7% similarity); and both strains clustered together in phylogenetic analysis. The genome of strain CBA3637(T) was found to consist of a single chromosome (3.51 Mbp; 36.98% G + C content). Comparative genomic analysis of the strain CBA3637(T) with A. butyrica DSM 103574(T) revealed that strain CBA3637(T) possessed five unique pathways related to polyamine biosynthesis, lipopolysaccharide metabolism, pyrimidine metabolism, and cofactor and vitamin metabolism. Strain CBA3637(T) contained C-14:0, C-16:0, and C-18:1 omega 9c as the major fatty acids, and diphosphatidylglycerol as the major polar lipid. No respiratory quinone was observed. Biochemical, chemotaxonomic, and genotypic data revealed that the strain CBA3637(T) is a representative of a novel species within the genus Aminipila, for which the name Aminipila terrae is proposed. The type strain is CBA3637(T) (= KACC 21651(T) = DSM 110662(T)).