Folpet induces mitochondrial dysfunction and ROS-mediated apoptosis in mouse Sertoli cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ham, Jiyeon | - |
dc.contributor.author | Yun, Bo Hyun | - |
dc.contributor.author | Lim, Whasun | - |
dc.contributor.author | Song, Gwonhwa | - |
dc.date.accessioned | 2022-02-26T18:41:11Z | - |
dc.date.available | 2022-02-26T18:41:11Z | - |
dc.date.created | 2022-02-07 | - |
dc.date.issued | 2021-08 | - |
dc.identifier.issn | 0048-3575 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/137045 | - |
dc.description.abstract | Folpet is a phthalimide type of fungicide and has been used to control several crop diseases. Although it has adverse effects on the gastrointestinal tract, its mechanism and toxic effects on testis have not been demonstrated. In the present study, we elucidated the cytotoxic effect of folpet on the mouse Sertoli cell line, TM4. Our results revealed that folpet suppressed viability and proliferative capacity of TM4 cells and further inhibited 3D spheroid formation. Moreover, folpet impeded appropriate cell cycle progression and induced apoptotic cell death in TM4 cells. It disrupted the electrochemical gradient of mitochondria and calcium homeostasis in TM4 cells. Furthermore, endoplasmic reticulum stress-related proteins were activated in folpet-treated TM4 cells, and relative reactive oxygen species (ROS) production was also increased. N-acetylcysteine (NAC) treatment reinstated the folpet-induced ROS generation in TM4 cells. Additionally, NAC restored the proliferative capacity and reduced the apoptotic cells in folpet-treated TM4 cells. Collectively, we demonstrated that folpet causes ROSmediated apoptotic cell death with mitochondrial dysfunction and calcium dysregulation in TM4 cells. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | ENDOPLASMIC-RETICULUM | - |
dc.subject | SPERMATOGENIC CELLS | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | ATP PRODUCTION | - |
dc.subject | CALCIUM | - |
dc.subject | GERM | - |
dc.subject | PROLIFERATION | - |
dc.subject | RELEVANCE | - |
dc.subject | TESTIS | - |
dc.title | Folpet induces mitochondrial dysfunction and ROS-mediated apoptosis in mouse Sertoli cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Gwonhwa | - |
dc.identifier.doi | 10.1016/j.pestbp.2021.104903 | - |
dc.identifier.scopusid | 2-s2.0-85108080087 | - |
dc.identifier.wosid | 000687339900005 | - |
dc.identifier.bibliographicCitation | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, v.177 | - |
dc.relation.isPartOf | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY | - |
dc.citation.title | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY | - |
dc.citation.volume | 177 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Entomology | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Entomology | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.subject.keywordPlus | ATP PRODUCTION | - |
dc.subject.keywordPlus | CALCIUM | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM | - |
dc.subject.keywordPlus | GERM | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | RELEVANCE | - |
dc.subject.keywordPlus | SPERMATOGENIC CELLS | - |
dc.subject.keywordPlus | TESTIS | - |
dc.subject.keywordAuthor | Folpet | - |
dc.subject.keywordAuthor | Fungicides | - |
dc.subject.keywordAuthor | Mitochondria | - |
dc.subject.keywordAuthor | Oxidative stress | - |
dc.subject.keywordAuthor | ROS | - |
dc.subject.keywordAuthor | Sertoli cell | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.