Single-stranded DNA probe paired aptasensor with extra dye binding sites to enhance its fluorescence response in the presence of a target compound
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Seo Won | - |
dc.contributor.author | Lim, Hyun Jeong | - |
dc.contributor.author | Chua, Beelee | - |
dc.contributor.author | Son, Ahjeong | - |
dc.date.accessioned | 2022-02-28T18:41:41Z | - |
dc.date.available | 2022-02-28T18:41:41Z | - |
dc.date.created | 2022-02-09 | - |
dc.date.issued | 2021-06-30 | - |
dc.identifier.issn | 2046-2069 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/137282 | - |
dc.description.abstract | The purpose of this study is to investigate the possibility of improving the performance of a DNA binding dye water quenching based aptasensor without changing or truncating the aptamer. To demonstrate the possibility of increasing the change in fluorescence of the aptasensor by pairing it with a suitable ssDNA probe, three ssDNA probes (probe 1, 2, and 3) were employed and the fluorescence from the bound dyes was measured. This showed that ssDNA probe 2 created the most additional binding sites. By varying the target compound concentration (0, 0.05, 0.5, 5, 50, and 500 mg L-1 4-n-nonylphenol), the corresponding change in the fluorescence signal of the unpaired and ssDNA probe paired aptasensors were measured and compared over a range of emission wavelengths. The response of all three ssDNA probe paired aptasensors showed good fit (R-2 = 0.88-0.92) to a logarithmic response. The sensitivity of the aptasensor paired with ssDNA probe 2 was improved by similar to 60%, whereas that of the aptasensor paired with ssDNA probe 3 was only improved by a marginal similar to 3%. This study is a demonstration of using an appropriate ssDNA probe to increase the number of binding sites and hence the performance of a DNA binding dye and water quenched aptasensor. It is a possibility that can be extended to similar aptasensors without having to change or truncate the aptamer. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | BISPHENOL-A | - |
dc.subject | SELECTIVE DETECTION | - |
dc.subject | APTAMERS | - |
dc.subject | BIOSENSORS | - |
dc.subject | ASSAY | - |
dc.title | Single-stranded DNA probe paired aptasensor with extra dye binding sites to enhance its fluorescence response in the presence of a target compound | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chua, Beelee | - |
dc.identifier.doi | 10.1039/d1ra00971k | - |
dc.identifier.scopusid | 2-s2.0-85108683047 | - |
dc.identifier.wosid | 000665644000056 | - |
dc.identifier.bibliographicCitation | RSC ADVANCES, v.11, no.35, pp.21796 - 21804 | - |
dc.relation.isPartOf | RSC ADVANCES | - |
dc.citation.title | RSC ADVANCES | - |
dc.citation.volume | 11 | - |
dc.citation.number | 35 | - |
dc.citation.startPage | 21796 | - |
dc.citation.endPage | 21804 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | APTAMERS | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | BIOSENSORS | - |
dc.subject.keywordPlus | BISPHENOL-A | - |
dc.subject.keywordPlus | SELECTIVE DETECTION | - |
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