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Arabidopsis TCX8 functions as a senescence modulator by regulating LOX2 expression

Authors
Noh, MinsooShin, Jin SeokHong, Jong ChanKim, Soo YounShin, Jeong Sheop
Issue Date
4월-2021
Publisher
SPRINGER
Keywords
Arabidopsis thaliana; LOX2; Senescence; TCP20; TCP4; TCX8
Citation
PLANT CELL REPORTS, v.40, no.4, pp.677 - 689
Indexed
SCIE
SCOPUS
Journal Title
PLANT CELL REPORTS
Volume
40
Number
4
Start Page
677
End Page
689
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/137670
DOI
10.1007/s00299-021-02663-y
ISSN
0721-7714
Abstract
Key message TCX8 localizes to nucleus and has transcriptional repression activity. TCX8 binds to the promoter region of LOX2 encoding lipoxygenase, causing JA biosynthesis suppression, and thereby delays plant senescence. Conserved CXC domain-containing proteins are found in most eukaryotes. Eight TCX proteins, which are homologs of animal CXC-Hinge-CXC (CHC) proteins, were identified in Arabidopsis, and three of them, TSO1, TCX2/SOL2 and TCX3/SOL1, have been reported to affect cell-cycle control. TCX8, one of the TCX family proteins, was believed to be a TF but its precise function has not been reported. Yeast two-hybrid screening revealed TCP20, a TF that binds to the promoter of LOX2 encoding lipoxygenase, as a strong candidate for interaction with TCX8. We confirmed that TCX8 directly interacts with TCP20 using in vitro pull-down assay and in vivo BiFC and observed that TCX8, as a TF, localizes to nucleus. Using EMSA and by analyzing phenotypes of TCX8-overexpression lines, we demonstrated that TCX8 regulates the expression of LOX2 by binding to either cis-element of LOX2 promoter to which TCP20 or TCP4 binds, affecting JA biosynthesis, and thereby delaying plant senescence. Our study provides new information about the role of TCX8 in modulating plant senescence through regulating LOX2 expression.
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