Novosphingobium aureum sp. nov., a marine bacterium isolated from salt flat sediment

Abstract

A Gram-stain-negative, aerobic, pale yellow-coloured, rod-shaped marine bacterium designated strain YJ-S2-02(T) was isolated from salt flat sediment sampled in Yongyu-do, Republic of Korea. Strain YJ-S2-02(T) grew at pH 6.0-9.0 (optimum, pH 7.0), 10-40 degrees C (optimum, 30 degrees C) and with optimum 1% (w/v) NaCl. The 16S rRNA gene sequence analysis indicated that strain YJ-S2-02(T) was closely related to Novosphingobium naphthalenivorans NBRC 102051(T)(97.8%) followed by Novosphingobium mathurense SM117(T) (97.5%), Novosphingobium indicum H25(T) (97.3%), Novosphingobium pentaromativorans US6-1(T) (96.8%), Novosphingobium fontis STM-14(T) (96.6%), Novosphingobium endophyticum EGI60015(T) (96.5%). Novosphingobium naphthae D39(T) (96.5%) and Novosphingobium malaysiense MUSC 273(T) (95.9%). The average nucleotide identity and estimated DNA-DNA hybridization values between YJ-S2-02(T) and related type strains were 77.0-77.9% and 19.1-24.0%.Strain YJ-52-02(T) was characterized as having Q-10 as the predominant respiratory quinone and the principal fatty acids (>10%) were summed feature 8 (C-18:1 omega 6c/w7c, 20.7%), C-18:1 omega 6c (16.3%) and C-17.1 omega 6c (11.8%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sphingolipids and two unidentified lipids. The DNA G+C content of strain YJ-S2-02(T) was 65.6 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, YJ-S2-02(T) should be classified as representing a novel species within the genus Novosphingobium, for which name Novosphingobium aureum is proposed, with the type strain YJ-S2-02(T) (=KACC 2167(T)=KCTC 72891(T)=JCM 33996(T)).