Polymerase II-Associated Factor 1 Complex-Regulated FLOWERING LOCUS C-Clade Genes Repress Flowering in Response to Chilling
DC Field | Value | Language |
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dc.contributor.author | Nasim, Zeeshan | - |
dc.contributor.author | Susila, Hendry | - |
dc.contributor.author | Jin, Suhyun | - |
dc.contributor.author | Youn, Geummin | - |
dc.contributor.author | Ahn, Ji Hoon | - |
dc.date.accessioned | 2022-03-14T06:42:03Z | - |
dc.date.available | 2022-03-14T06:42:03Z | - |
dc.date.created | 2022-03-14 | - |
dc.date.issued | 2022-02-09 | - |
dc.identifier.issn | 1664-462X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/138917 | - |
dc.description.abstract | RNA polymerase II-associated factor 1 complex (PAF1C) regulates the transition from the vegetative to the reproductive phase primarily by modulating the expression of FLOWERING LOCUS C (FLC) and FLOWERING LOCUS M [FLM, also known as MADS AFFECTING FLOWERING1 (MAF1)] at standard growth temperatures. However, the role of PAF1C in the regulation of flowering time at chilling temperatures (i.e., cold temperatures that are above freezing) and whether PAF1C affects other FLC-clade genes (MAF2-MAF5) remains unknown. Here, we showed that Arabidopsis thaliana mutants of any of the six known genes that encode components of PAF1C [CELL DIVISION CYCLE73/PLANT HOMOLOGOUS TO PARAFIBROMIN, VERNALIZATION INDEPENDENCE2 (VIP2)/EARLY FLOWERING7 (ELF7), VIP3, VIP4, VIP5, and VIP6/ELF8] showed temperature-insensitive early flowering across a broad temperature range (10 degrees C-27 degrees C). Flowering of PAF1C-deficient mutants at 10 degrees C was even earlier than that in flc, flm, and flc flm mutants, suggesting that PAF1C regulates additional factors. Indeed, RNA sequencing (RNA-Seq) of PAF1C-deficient mutants revealed downregulation of MAF2-MAF5 in addition to FLC and FLM at both 10 and 23 degrees C. Consistent with the reduced expression of FLC and the FLC-clade members FLM/MAF1 and MAF2-MAF5, chromatin immunoprecipitation (ChIP)-quantitative PCR assays showed reduced levels of the permissive epigenetic modification H3K4me3/H3K36me3 and increased levels of the repressive modification H3K27me3 at their chromatin. Knocking down MAF2-MAF5 using artificial microRNAs (amiRNAs) in the flc flm background (35S::amiR-MAF2-5 flc flm) resulted in significantly earlier flowering than flc flm mutants and even earlier than short vegetative phase (svp) mutants at 10 degrees C. Wild-type seedlings showed higher accumulation of FLC and FLC-clade gene transcripts at 10 degrees C compared to 23 degrees C. Our yeast two-hybrid assays and in vivo co-immunoprecipitation (Co-IP) analyses revealed that MAF2-MAF5 directly interact with the prominent floral repressor SVP. Late flowering caused by SVP overexpression was almost completely suppressed by the elf7 and vip4 mutations, suggesting that SVP-mediated floral repression required a functional PAF1C. Taken together, our results showed that PAF1C regulates the transcription of FLC and FLC-clade genes to modulate temperature-responsive flowering at a broad range of temperatures and that the interaction between SVP and these FLC-clade proteins is important for floral repression. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | FRONTIERS MEDIA SA | - |
dc.subject | MADS-BOX GENE | - |
dc.subject | WINTER-ANNUAL HABIT | - |
dc.subject | PAF1 COMPLEX | - |
dc.subject | HISTONE METHYLATION | - |
dc.subject | PHENOTYPIC ANALYSIS | - |
dc.subject | TIME GENES | - |
dc.subject | ARABIDOPSIS | - |
dc.subject | TRANSCRIPTION | - |
dc.subject | PROTEIN | - |
dc.subject | FLC | - |
dc.title | Polymerase II-Associated Factor 1 Complex-Regulated FLOWERING LOCUS C-Clade Genes Repress Flowering in Response to Chilling | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ahn, Ji Hoon | - |
dc.identifier.doi | 10.3389/fpls.2022.817356 | - |
dc.identifier.scopusid | 2-s2.0-85125132879 | - |
dc.identifier.wosid | 000760671600001 | - |
dc.identifier.bibliographicCitation | FRONTIERS IN PLANT SCIENCE, v.13 | - |
dc.relation.isPartOf | FRONTIERS IN PLANT SCIENCE | - |
dc.citation.title | FRONTIERS IN PLANT SCIENCE | - |
dc.citation.volume | 13 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.subject.keywordPlus | MADS-BOX GENE | - |
dc.subject.keywordPlus | WINTER-ANNUAL HABIT | - |
dc.subject.keywordPlus | PAF1 COMPLEX | - |
dc.subject.keywordPlus | HISTONE METHYLATION | - |
dc.subject.keywordPlus | PHENOTYPIC ANALYSIS | - |
dc.subject.keywordPlus | TIME GENES | - |
dc.subject.keywordPlus | ARABIDOPSIS | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | FLC | - |
dc.subject.keywordAuthor | Arabidopsis | - |
dc.subject.keywordAuthor | FLC | - |
dc.subject.keywordAuthor | MAFs | - |
dc.subject.keywordAuthor | PAF1C | - |
dc.subject.keywordAuthor | flowering | - |
dc.subject.keywordAuthor | epigenetics | - |
dc.subject.keywordAuthor | temperature | - |
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