A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development
DC Field | Value | Language |
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dc.contributor.author | Reyes-Alcaraz, Arfaxad | - |
dc.contributor.author | Lucero Garcia-Rojas, Emilio Y. | - |
dc.contributor.author | Merlinsky, Elizabeth A. | - |
dc.contributor.author | Seong, Jae Young | - |
dc.contributor.author | Bond, Richard A. | - |
dc.contributor.author | McConnell, Bradley K. | - |
dc.date.accessioned | 2022-04-01T07:40:42Z | - |
dc.date.available | 2022-04-01T07:40:42Z | - |
dc.date.created | 2022-04-01 | - |
dc.date.issued | 2022-03-08 | - |
dc.identifier.issn | 2399-3642 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/139336 | - |
dc.description.abstract | Internalization of membrane proteins plays a key role in many physiological functions; however, highly sensitive and versatile technologies are lacking to study such processes in real-time living systems. Here we describe an assay based on bioluminescence able to quantify membrane receptor trafficking for a wide variety of internalization mechanisms such as GPCR internalization/recycling, antibody-mediated internalization, and SARS-CoV2 viral infection. This study represents an alternative drug discovery tool to accelerate the drug development for a wide range of physiological processes, such as cancer, neurological, cardiopulmonary, metabolic, and infectious diseases including COVID-19. Membrane protein trafficking is monitored using split nanoluciferase. Receptor internalization leads to complementation on the early endosome and a bioluminescent response, and is applied to receptor internalization/recycling, antibody-mediated internalization and SARS-CoV2 entry. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE PORTFOLIO | - |
dc.subject | RECEPTOR INTERNALIZATION | - |
dc.subject | COMPOUND HETEROZYGOSITY | - |
dc.subject | IN-SITU | - |
dc.subject | MUTATIONS | - |
dc.subject | CCR5 | - |
dc.title | A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Seong, Jae Young | - |
dc.identifier.doi | 10.1038/s42003-022-03163-9 | - |
dc.identifier.scopusid | 2-s2.0-85126077149 | - |
dc.identifier.wosid | 000766191500003 | - |
dc.identifier.bibliographicCitation | COMMUNICATIONS BIOLOGY, v.5, no.1 | - |
dc.relation.isPartOf | COMMUNICATIONS BIOLOGY | - |
dc.citation.title | COMMUNICATIONS BIOLOGY | - |
dc.citation.volume | 5 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | RECEPTOR INTERNALIZATION | - |
dc.subject.keywordPlus | COMPOUND HETEROZYGOSITY | - |
dc.subject.keywordPlus | IN-SITU | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.subject.keywordPlus | CCR5 | - |
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