Escherichia coli inner membrane display system for high-throughput screening of dimeric proteins
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정상택 | - |
dc.date.accessioned | 2022-04-09T03:40:59Z | - |
dc.date.available | 2022-04-09T03:40:59Z | - |
dc.date.created | 2022-04-08 | - |
dc.date.issued | 2018-12 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/139713 | - |
dc.description.abstract | Multimer formation is indispensable to the intrinsicbiologicalfunctions of many natural proteins. For example, the human immunoglobulin G (IgG) antibody has two variable regions (heavy chain variable domain [VH] and light chain variable domain [VL]) that must be assembled for specific antigen binding, and homodimerization of the antibody's Fc domain is essential for eliciting therapeutic effector functions. For the more efficient high-throughput directed evolution of multimeric proteins with ease of cultivation and handling, here we report a membrane protein drift and assembly (MPDA) system, in which a multimeric protein is displayed on a bacterial inner membrane by drifting and auto-assembling membrane-anchored subunit polypeptides. This system enabled the auto-assembly of membrane-tethered Fv domains (VH and VL) or the monomeric Fc domain into a functional hetero- or homodimeric protein complex on the bacterial inner membrane. This system could also be used to enrich a desired engineered Fc variant from a mixture containing a million-fold excess of wild-type Fc domain, indicating the applicability of the MPDA system for the high-throughput directed evolution of a variety of multimeric proteins, such as cytokines, enzymes, or structural proteins. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Escherichia coli inner membrane display system for high-throughput screening of dimeric proteins | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | 정상택 | - |
dc.identifier.doi | 10.1002/bit.26826 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOENGINEERING, v.115, no.12, pp.2849 - 2858 | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.citation.title | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.citation.volume | 115 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 2849 | - |
dc.citation.endPage | 2858 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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