Engineered aglycosylated full-length IgG Fc variants exhibiting improved Fc gamma RIIIa binding and tumor cell clearance
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정상택 | - |
dc.date.accessioned | 2022-04-09T16:40:19Z | - |
dc.date.available | 2022-04-09T16:40:19Z | - |
dc.date.created | 2022-04-08 | - |
dc.date.issued | 2018-01 | - |
dc.identifier.issn | 19420862 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/139797 | - |
dc.description.abstract | Fc gamma RIIIa, which is predominantly expressed on the surface of natural killer cells, plays a key role in antibody-dependent cell-mediated cytotoxicity ( ADCC), a major effector function of therapeutic IgG antibodies that results in the death of aberrant cells. Despite the potential uses of aglycosylated IgG antibodies, which can be easily produced in bacteria and do not have complicated glycan heterogeneity issues, they show negligible binding to FcgRIIIa and abolish the activation of immune leukocytes for tumor cell clearance, in sharp contrast to most glycosylated IgG antibodies used in the clinical setting. For directed evolution of aglycosylated Fc variants that bind to FcgRIIIa and, in turn, exert potent ADCC effector function, we randomized the aglycosylated Fc region of full-length IgG expressed on the inner membrane of Escherichia coli. Multiple rounds of high-throughput screening using flow cytometry facilitated the isolation of aglycosylated IgG Fc variants that exhibited higher binding affinity to Fc gamma RIIIa-158V and Fc gamma RIIIa-158F compared with clinical-grade trastuzumab (Herceptin (R)). The resulting aglycosylated trastuzumab IgG antibody Fc variants could elicit strong peripheral blood mononuclear cell-mediated ADCC without glycosylation in the Fc regio | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR FRANCIS INC | - |
dc.title | Engineered aglycosylated full-length IgG Fc variants exhibiting improved Fc gamma RIIIa binding and tumor cell clearance | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | 정상택 | - |
dc.identifier.doi | 10.1080/19420862.2017.1402995 | - |
dc.identifier.bibliographicCitation | MABS, v.10, no.2, pp.278 - 289 | - |
dc.relation.isPartOf | MABS | - |
dc.citation.title | MABS | - |
dc.citation.volume | 10 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 278 | - |
dc.citation.endPage | 289 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Aglycosylated IgG | - |
dc.subject.keywordAuthor | Antibody-dependent cell-mediated cytotoxicity | - |
dc.subject.keywordAuthor | Effector functions | - |
dc.subject.keywordAuthor | Fc engineering | - |
dc.subject.keywordAuthor | Fc gamma RIIIa | - |
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